NDLI: Calcium-dependent regulation of the cell cycle via a novel MAPK–NF-κB pathway in Swiss 3T3 cells

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Calcium-dependent regulation of the cell cycle via a novel MAPK–NF-κB pathway in Swiss 3T3 cells

Content Provider	Semantic Scholar
Author	See, V. Rajala, Nina K. M. Spiller, David G. White, Michael R. H.
Copyright Year	2004
Abstract	Nuclear factor kappa B (NF-kappaB) has been implicated in the regulation of cell proliferation and transformation. We investigated the role of the serum-induced intracellular calcium increase in the NF-kappaB--dependent cell cycle progression in Swiss 3T3 fibroblasts. Noninvasive photoactivation of a calcium chelator (Diazo-2) was used to specifically disrupt the transient rise in calcium induced by serum stimulation of starved Swiss 3T3 cells. The serum-induced intracellular calcium peak was essential for subsequent NF-kappaB activation (measured by real-time imaging of the dynamic p65 and IkappaBalpha fluorescent fusion proteins), cyclin D1 (CD1) promoter-directed transcription (measured by real-time luminescence imaging of CD1 promoter-directed firefly luciferase activity), and progression to cell division. We further showed that the serum-induced mitogen-activated protein kinase (MAPK) phosphorylation is calcium dependent. Inhibition of the MAPK- but not the PtdIns3K-dependent pathway inhibited NF-kappaB signaling, and further, CD1 transcription and cell cycle progression. These data suggest that a serum-dependent calcium signal regulates the cell cycle via a MAPK--NF-kappaB pathway in Swiss 3T3 cells.
Starting Page	661
Ending Page	672
Page Count	12
File Format	PDF HTM / HTML
PubMed reference number	15326199
Volume Number	166
Journal	The Journal of cell biology
Alternate Webpage(s)	http://jcb.rupress.org/content/jcb/166/5/661.full.pdf
Language	English
Access Restriction	Open
Subject Keyword	3T3 Cells Calcium Cell Cycle Progression Cell Proliferation Cell division Cyclin D1 Firefly Family Leukemia, B-Cell Luciferases Luciferases, Firefly Mitogen-Activated Protein Kinases Mitogens NF-kappa B eIF-2 Kinase luminescence
Content Type	Text
Resource Type	Article

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