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A fluorescent light-up probe with "AIE + ESIPT" characteristics for specific detection of lysosomal esterase.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Gao, Meng Hu, Qinglian Feng, Guangxue Tang, Ben Zhong |
| Copyright Year | 2014 |
| Abstract | We report a fluorescent light-up probe AIE-Lyso-1 for in situ visualization of lysosomal esterase activity. The probe is based on a salicyladazine fluorogen, which is conjugated with esterase reactive acetoxyl groups and lysosome-targeting morpholine moieties. The probe has characteristics of both aggregation-induced emission (AIE) and excited-state intramolecular proton transfer (ESIPT), which shows significant advantages, such as lysosome-specific targeting, no self-quenching at high concentration, excellent light-up ratio, large Stokes shift, low cytotoxicity, and high specificity to esterase. It has also been used for in situ monitoring of lysosomal esterase activity and tracking lysosomal movements in living cells, which has great potential for the diagnosis of Wolman disease caused by deficiency of lysosomal esterase. |
| Starting Page | 3438 |
| Ending Page | 3442 |
| Page Count | 5 |
| File Format | PDF HTM / HTML |
| DOI | 10.1039/C4TB00345D |
| PubMed reference number | 32261463 |
| Journal | Medline |
| Volume Number | 2 |
| Issue Number | 22 |
| Alternate Webpage(s) | http://www.rsc.org/suppdata/tb/c4/c4tb00345d/c4tb00345d1.pdf |
| Alternate Webpage(s) | https://pubs.rsc.org/en/content/getauthorversionpdf/C4TB00345D |
| Journal | Journal of materials chemistry. B |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
