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The structure of the core NuRD repression complex provides insights into its interaction with chromatin
| Content Provider | Semantic Scholar |
|---|---|
| Author | Millard, Christopher J. Varma, Niranjan Saleh, Almutasem Morris, Kyle L. Watson, Peter James Bottrill, Andrew R. Fairall, Louise Smith, Corinne J. I. Schwabe, John W. R. |
| Copyright Year | 2016 |
| Abstract | The NuRD complex is a multi-protein transcriptional corepressor that couples histone deacetylase and ATP-dependent chromatin remodelling activities. The complex regulates the higher-order structure of chromatin, and has important roles in the regulation of gene expression, DNA damage repair and cell differentiation. HDACs 1 and 2 are recruited by the MTA1 corepressor to form the catalytic core of the complex. The histone chaperone protein RBBP4, has previously been shown to bind to the carboxy-terminal tail of MTA1. We show that MTA1 recruits a second copy of RBBP4. The crystal structure reveals an extensive interface between MTA1 and RBBP4. An EM structure, supported by SAXS and crosslinking, reveals the architecture of the dimeric HDAC1:MTA1:RBBP4 assembly which forms the core of the NuRD complex. We find evidence that in this complex RBBP4 mediates interaction with histone H3 tails, but not histone H4, suggesting a mechanism for recruitment of the NuRD complex to chromatin. |
| Starting Page | 787 |
| Ending Page | 795 |
| Page Count | 9 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://wrap.warwick.ac.uk/78715/1/WRAP_elife-13941-v1-download.pdf |
| Alternate Webpage(s) | http://ftp.ncbi.nlm.nih.gov/pub/pmc/29/a9/elife-13941.PMC4841774.pdf |
| PubMed reference number | 27098840v1 |
| Alternate Webpage(s) | https://doi.org/10.7554/eLife.13941 |
| DOI | 10.7554/elife.13941 |
| Journal | eLife |
| Volume Number | 5 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Carboxyl Group Catalytic Domain Cell Differentiation process Cross link DNA Repair Gene Expression Regulation Histone Deacetylase Histone H3 Histones Influenza virus A H4 Ab:Titr:Pt:Ser:Qn Interface Device Component MTA1 gene Molecular Chaperones Numerous RBBP4 gene Repression, Psychology Tail Transcription Repressor/Corepressor Transcription, Genetic chromatin remodeling |
| Content Type | Text |
| Resource Type | Article |
