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A liposomal peptide vaccine inducing CD8+ T cells in HLA-A2.1 transgenic mice, which recognise human cells encoding hepatitis C virus (HCV) proteins.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Engler, Olivier B. Schwendener, Reto Albert Dai, Wen Juan Wölk, Benno Pichler, Werner Moradpour, Darius Brunner, Thomas Levin Cerny, Andreas |
| Copyright Year | 2004 |
| Abstract | Virus specific T cell responses play an important role in resolving acute hepatitis C virus (HCV) infections. Using the HLA-A2.1 transgenic mouse model we investigated the potential of a liposomal peptide vaccine to prime a CD8(+) T cell response against 10 different HCV epitopes, relevant for human applications. We were able to demonstrate the induction of strong cytotoxic T cell responses and high numbers of IFN-gamma-secreting cells, which persisted at high levels for at least 3 months. Co-integrating CpG oligonucleotides into liposomes further increased the number of IFN-gamma-secreting cells by 2-10-fold for most epitopes tested. The frequency of specific cells was further analysed with chimeric A2 tetramers bearing the NS31073-1081 epitope and was estimated at 2-23% of the CD8(+) T cell population. Importantly, mouse effector cells, specific for this epitope, were also capable of lysing a human target cell line expressing HCV proteins. This finding and the specific protection observed in challenge experiments with recombinant vaccinia virus expressing HCV sequences emphasise the biological relevance of the vaccine-induced immune response. In conclusion, such liposome formulations represent a safe and promising strategy to stimulate the CD8(+) T cell against HCV. |
| Starting Page | 58 |
| Ending Page | 68 |
| Page Count | 11 |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/j.vaccine.2004.05.009 |
| PubMed reference number | 15519708 |
| Journal | Medline |
| Volume Number | 23 |
| Issue Number | 1 |
| Alternate Webpage(s) | https://kops.uni-konstanz.de/bitstream/handle/123456789/14287/2004_Engler-Vaccine_04.pdf?isAllowed=y&sequence=2 |
| Alternate Webpage(s) | http://kops.uni-konstanz.de/bitstream/handle/123456789/14287/2004_Engler-Vaccine_04.pdf?isAllowed=y&sequence=2 |
| Alternate Webpage(s) | http://www.imcr.uzh.ch/static/onkwww/vaccine2.pdf |
| Alternate Webpage(s) | https://doi.org/10.1016/j.vaccine.2004.05.009 |
| Journal | Vaccine |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
