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Conjugated linoleic acid increases Ucp1 and activated AMPK in adipose tissue
| Content Provider | Semantic Scholar |
|---|---|
| Author | Wendel, Angela A. |
| Copyright Year | 2007 |
| Abstract | lipogenesis oxidation * Experimental design. 6-week old, male B6.V-Lepob/OlaHsd (ob/ob) mice were obtained through Harlan (Indianapolis, IN) and housed 4/cage at 22oC ± 0.5oC on a 12-hour light/dark cycle. Mice were maintained on isocaloric, modified AIN93G diets (Bio-Serv, Frenchtown, NJ) containing 6.5% fat. Mice received diets that contained either 6.5% soybean oil (CON; n=8) or 5% soybean oil and 1.5% CLA mixed triglycerides (CLA; n=8). CLA mixed triglycerides (Tonalin TG 80, Cognis Corp., Cincinnati, OH) were ~80% CLA composed of 39.2% c9t11and 38.5% t10c12-CLA isomers. At 4 weeks, after an overnight (12 hr) fast, mice were anesthetized with isoflurane and blood was collected via cardiac puncture. Tissues were quickly harvested, weighed, snap-frozen with liquid nitrogen, and stored at -80oC until analyses. All procedures were in accordance with institution guidelines and approved by the Institutional Animal Care and Use Committee of The Ohio State University. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://kb.osu.edu/bitstream/handle/1811/24800/OARDC%202007.pdf?isAllowed=y&sequence=1 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
