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9-cis retinoic acid induces monocyte chemoattractant protein-1 secretion in human monocytic THP-1 cells.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Bisgaier, Charles L. Aviram, Michael Newton, Roger Schofield |
| Copyright Year | 1999 |
| Abstract | Monocyte migration and activation are regulated by monocyte chemoattractant protein-1 (MCP-1). Prior studies have shown MCP-1 expression is modulated by a variety of ligands that act through extracellular receptors. In the current study, we show 9-cis retinoic acid (RA), a ligand for the nuclear hormone receptor retinoid X receptor (RXR) and retinoic acid receptor (RAR), markedly induces the expression of MCP-1. In human THP-1 monocytic leukemia cells cultured with RA (0.05 to 500 nmol/L), MCP-1 expression was induced rapidly, significantly, and dose-dependently by as much as 165-fold. MCP-1 RNA level was also increased in RA-treated cells. Expression of PPARgamma, a heterodimer partner of RXR, is also markedly induced by RA in THP-1 cells. However, BRL49653, a PPARgamma ligand, failed to induce MCP-1 secretion either alone or to modify the expression level induced by RA. In contrast, BRL49653 significantly increased MCP-1 (biotinylated MCP-1) binding to THP-1 cells, whereas RA had no effect. Other peroxisome proliferator activated receptor (PPAR) ligands, 15d-PGJ(2) and troglitazone (PPARgamma), Wy14,643 (PPARalpha), and PD195599 (PPARbeta) inhibited the induction of MCP-1 by RA. RA's effect on MCP-1 expression in human elutriated monocytes were similar to that observed in the THP-1 cells. These studies identify RA as a nuclear signal for MCP-1 induction in undifferentiated human monocytic cells. These studies also suggest monocyte MCP-1 expression induced through RA may modulate cell migration. |
| File Format | PDF HTM / HTML |
| DOI | 10.1161/01.ATV.19.9.2105 |
| PubMed reference number | 10479651 |
| Journal | Medline |
| Volume Number | 19 |
| Issue Number | 9 |
| Alternate Webpage(s) | http://atvb.ahajournals.org/content/atvbaha/19/9/2105.full.pdf |
| Alternate Webpage(s) | http://atvb.ahajournals.org/content/atvbaha/19/9/2105.full.pdf?download=true |
| Alternate Webpage(s) | https://doi.org/10.1161/01.ATV.19.9.2105 |
| Journal | Arteriosclerosis, thrombosis, and vascular biology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
