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Enhanced expression of a mosquito larvicidal gene(s) of Bacillus sphaericus 1593M in Escherichia coli
| Content Provider | Semantic Scholar |
|---|---|
| Author | Rajamohan, Francis Suresh, Gopalakrishnan Jayaraman, Kunthala |
| Copyright Year | 1992 |
| Abstract | Bacillus sphaericus is known for its potential as a biocontrol agent to Cuhx and Anopheles species of mosquitoes. A 3.6-kb-DNA fragment of B. sphaericus IS93M encoding mosquito larvicidal activity has earlier been cloned by us in £ coli, using the vector pBR322 and the recombinant had a low larvicidal activity. This fragment has now been recloned into an expression vector of E coli, pPR683. The resultant recombinant clone expressed high levels of larvicidal factors under the control of tac promoter at levels comparable to that of B. sphaericus 1593M. Since lactose can be an effective inducer of the larvicidal factors in this recombinant, an economic production of bhmsecticidai factors is forecasted. |
| Starting Page | 205 |
| Ending Page | 208 |
| Page Count | 4 |
| File Format | PDF HTM / HTML |
| Volume Number | 63 |
| Alternate Webpage(s) | http://shodhganga.inflibnet.ac.in:8080/jspui/bitstream/10603/73936/17/17_publication%20details.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
