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The influence of amino acids on the lobeline production of Lobelia inflata L. hairy root cultures
| Content Provider | Semantic Scholar |
|---|---|
| Author | Bálványos, István Szőke, Éva Kursinszki, L. |
| Copyright Year | 2004 |
| Abstract | The herb Lobelia inflata L. (Lobeliaceae) containspiperidine alkaloids. The main alkaloid is the pharmacologically-activelobeline. We have studied the effects of alkaloid precursor amino acids (lysineand phenylalanine) on the growth and alkaloid production of hairy root culturesof Lobelia inflata L. The hairy root clone 8009/h7transformed with Agrobacterium rhizogenes strain R 1601wascultivated on B5 solid medium containing lysine (Lys) and phenylalanine (Phe)both in the presence and absence of the growth regulators IAA and kinetin. Onthe medium containing hormones growth was delayed until day 14. The applicationof growth regulators to the B5 media containing amino acids either singly or incombination increased the biomass in all cases. The maximum dry weight wasachieved in a medium containing Phe + Lys and growthregulators. The highest lobeline level (36 μg/g) was detectedintissues cultivated on hormone-free medium containing Phe. In hormone-freemedia,lobeline production increased in the presence of either Phe or Lys comparedwiththe control, but the addition of both greatly decreased synthesis. In contrast,the addition of both amino acids to media supplemented with IAA and kinetinincreased lobeline production. |
| Starting Page | 241 |
| Ending Page | 244 |
| Page Count | 4 |
| File Format | PDF HTM / HTML |
| DOI | 10.1023/A:1016574003070 |
| Volume Number | 36 |
| Alternate Webpage(s) | http://aok.pte.hu/docs/gyogyegy/file/citalasok_BotzL_o%CC%88sszes_121012.pdf |
| Alternate Webpage(s) | https://doi.org/10.1023/A%3A1016574003070 |
| Journal | Plant Growth Regulation |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
