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Quantitative analysis of the number of antigens immobilized on a glass surface by AFM.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Kim, Hyonchol Tsuruta, Satoshi Arakawa, Hideo Osada, Toshiya Ikai, Atsushi |
| Copyright Year | 2004 |
| Abstract | To develop force measurements using an atomic force microscope (AFM) in a quantitative manner, it is necessary to estimate the number density of target molecules on a sample surface, and for this, the sensitivity of detection should be known. In this study, the AFM was used as a mechanical detector and an antigen and its antibody were used as a model to evaluate the sensitivity of detection. Antigens were immobilized on a glass surface and number density was estimated by monitoring optical absorbance due to product formation by the reaction of crosslinkers. The concentration of antigen was controlled by mixing control peptides. A microbead was used as a probe and antibodies were immobilized on the bead. AFM force measurements were then made for a range of number densities in the order of 10-10(6) antigen molecules per square micrometer of surface and were compared to evaluate the sensitivity of detection. Our result establishes the reliability of estimating a number of molecules like receptors on the cell surface, and indicates that the AFM is useful as a mechanical detector with high sensitivity. |
| Starting Page | 35 |
| Ending Page | 40 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://tdl.libra.titech.ac.jp/hkshi/xc/contents/pdf/117076245/8 |
| PubMed reference number | 15231311v1 |
| Volume Number | 100 |
| Issue Number | 3-4 |
| Journal | Ultramicroscopy |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Detectors Estimated Microscope Device Component Microspheres Scanning Probe Microscopes (device) Spectrophotometry, Atomic Absorption density |
| Content Type | Text |
| Resource Type | Article |
