NDLI: Alkannin, HSP70 Inducer, Protects against UVB-Induced Apoptosis in Human Keratinocytes

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Alkannin, HSP70 Inducer, Protects against UVB-Induced Apoptosis in Human Keratinocytes

Content Provider	Semantic Scholar
Author	Yoshihisa, Yoko Hassan, Mariame Ali Furusawa, Yukihiro Tabuchi, Yoshiaki Kondo, Takashi Shimizu, Tadamichi
Copyright Year	2012
Abstract	Alkannin is an active constituent from the root extract of Alkanna tinctoria of the Boraginaceae family and it may have utility as a heat shock protein 70 (HSP70) inducer in living organisms. Here, the effects of alkannin-induced HSP70 on ultraviolet (UV) B (40 mJ/cm(2))-induced apoptosis were investigated in human keratinocyte HaCaT cells. Pretreatment of cells with alkannin (1 µM) caused significant inhibition of UVB-induced apoptosis and caspase-3 cleavage. On the other hand, the addition of KNK437 (HSP70 inhibitor) reversed the action of alkannin increasing UVB-induced apoptosis in a dose-dependent manner. In addition, differences in gene expression associated with the suppression of UVB-induced apoptosis in the presence of alkannin were investigated using Gene Chip assay. Our results indicate that alkannin suppresses UVB-induced apoptosis through the induction of HSP70 in human keratinocytes, and therefore, we suggest the usefulness of using alkannin as an antiaging agent.
File Format	PDF HTM / HTML
DOI	10.1371/journal.pone.0047903
PubMed reference number	23110127
Journal	Medline
Volume Number	7
Alternate Webpage(s)	http://ftp.ncbi.nlm.nih.gov/pub/pmc/fe/bf/pone.0047903.PMC3478268.pdf
Alternate Webpage(s)	http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0047903&type=printable
Journal	PloS one
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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