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Targeted CT imaging of human hepatocellular carcinoma using low-generation dendrimer-entrapped gold nanoparticles modified with lactobionic acid.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Cao, Yiyun Shen, Mingwu Xia, Jindong Shi, Xiangyang |
| Copyright Year | 2015 |
| Abstract | Development of cost-effective nanoscale contrast agents for targeted tumor computed tomography (CT) imaging remains a great challenge. Here, we report the synthesis of dendrimer-entrapped AuNPs (Au DENPs) using generation 2 poly(amidoamine) dendrimers pre-modified with fluorescein isothiocyanate via a thiourea linkage and lactobionic acid (LA) via a polyethylene glycol spacer as templates. The formed Au DENPs were characterized via different techniques and were used as a nanoprobe for targeted CT imaging of hepatocellular carcinoma (HCC). We show that the LA-modified Au DENPs with a mean Au core size of 1.8 nm are water-dispersible, colloidally stable under different temperatures (4-50 °C) and pH (5-8) conditions, and cytocompatible in the studied concentration range. Flow cytometry results reveal that the LA-Au DENPs are able to specifically target HepG2 cells (a human HCC cell line) overexpressing asialoglycoprotein receptors via a receptor-mediated targeting pathway. Importantly, the developed LA-Au DENPs can be used as a nanoprobe for targeted CT imaging of HepG2 cells in vitro and the xenografted tumor model in vivo. With the demonstrated organ compatibility, the developed LA-Au DENPs using low-generation dendrimers as templates can be a good candidate to be used as a highly efficient and cost-effective nanoprobe for targeted CT imaging of HCC. |
| Starting Page | 286 |
| Ending Page | 295 |
| Page Count | 10 |
| File Format | PDF HTM / HTML |
| DOI | 10.1039/C4TB01542H |
| Alternate Webpage(s) | http://www.rsc.org/suppdata/tb/c4/c4tb01542h/c4tb01542h1.pdf |
| PubMed reference number | 32261949 |
| Alternate Webpage(s) | https://doi.org/10.1039/C4TB01542H |
| Journal | Medline |
| Volume Number | 3 |
| Issue Number | 2 |
| Journal | Journal of materials chemistry. B |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
