Lipid vesicles as carriers for introducing materials into cultured cells: influence of vesicle lipid composition on mechanism(s) of vesicle incorporation into cells.

Content Provider	Semantic Scholar
Author	Poste, George Papahadjopoulos, Demetrios
Copyright Year	1976
Abstract	The mechanisms involved in the uptake of uni- and multi-lamellar lipid vesicles by BALB/c mouse 3T3 cells have been investigated. Vesicles are incorporated into cells both by endocytosis and by a nonendocytotic mechanism which we propose involves fusion of vesicles with the plasma membrane. The nonendocytotic pathway predominates in the uptake of negatively charged vesicles composed of phospholipids that are "fluid" (phosphatidylserine/phosphatidylcholine) at 37 degrees. Neutral fluid vesicles (phosphatidylcholine) and negatively charged vesicles prepared from "solid" phospholipids (phosphatidylserine/distearylphosphatidylcholine/dipalmitoylphosphatidylcholine) are instead incorporated largely by endocytosis. Uptake of the latter classes of vesicle is reduced (80-90% inhibition) by inhibitors of cellular energy metabolism and by cytochalasin B.
Starting Page	1
Ending Page	6
Page Count	6
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.pnas.org/content/73/5/1603.full.pdf
PubMed reference number	818640v1
Volume Number	73
Issue Number	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Language	English
Access Restriction	Open
Subject Keyword	1,2-Dipalmitoylphosphatidylcholine 3T3 Cells Charge (electrical) Class Cytochalasins Eighty Import into cell Numerous Phosphatidylcholines Phosphatidylserines Phospholipids Plasma membrane Vesicle (morphologic abnormality)
Content Type	Text
Resource Type	Article