NDLI: Heparin-binding proteins of seminal plasma in Nellore bulls

Content Provider	Semantic Scholar
Author	Souza-Netoiii, Carlos Eurico Fernandes I. Fabiana Ferreira De Souza I. I. Jaym Paulo Eduardo Martins Ribola I. V.
Copyright Year	2009
Abstract	The aim of this study was to identify heparinbinding proteins (HBPs) in seminal plasma of Nellore (Bos taurus indicus) bulls. Bulls (n=4), 30-36 months old, 500550kg with satisfactory seminal quality were selected. After the centrifugation, samples of the seminal plasma were pooled and the HBPs were isolated by heparin-affinity chromatography. The recovered HBPs fractions were pooled. One-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDSPAGE) 12.5% was performed in vertical minigels. Eight bands with molecular weights ranging from 15 to 63kDa were observed. Two proteins were identified (22 and 25kDa), similar to those previously described in Bos taurus taurus bulls. Other bands identified in this study (39, 53, 58 and 63kDa) have not been previously observed and possibly they are specific to Nellore semen.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.scielo.br/pdf/cr/v39n1/a44cr584.pdf
Language	English
Access Restriction	Open
Subject Keyword	Bands Bos taurus Cattle Centrifugation Chromatography, Affinity Gel Electrophoresis (lab technique) Molecular Weight Plasma Active Polyacrylamide Gel Electrophoresis Pooled Sample Processor affinity Sodium Dodecyl Sulfate Sol-gel Sulfate measurement heparin polyacrylamide gels
Content Type	Text
Resource Type	Article

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