Readily releasable pool of synaptic vesicles measured at single synaptic contacts.

Content Provider	Semantic Scholar
Author	Trigo, F. F. Sakaba, Takeshi Ogden, Dorothy Marty, Alain
Copyright Year	2012
Abstract	To distinguish between different models of vesicular release in brain synapses, it is necessary to know the number of vesicles of transmitter that can be released immediately at individual synapses by a high-calcium stimulus, the readily releasable pool (RRP). We used direct stimulation by calcium uncaging at identified, single-site inhibitory synapses to investigate the statistics of vesicular release and the size of the RRP. Vesicular release, detected as quantal responses in the postsynaptic neuron, showed an unexpected stochastic variation in the number of quanta from stimulus to stimulus at high intracellular calcium, with a mean of 1.9 per stimulus and a maximum of three or four. The results provide direct measurement of the RRP at single synaptic sites. They are consistent with models in which release proceeds from a small number of vesicle docking sites with an average occupancy around 0.7.
File Format	PDF HTM / HTML
DOI	10.1073/pnas.1209798109
PubMed reference number	23074252
Journal	Medline
Volume Number	109
Issue Number	44
Alternate Webpage(s)	http://www.pnas.org/content/109/44/18138.full.pdf
Journal	Proceedings of the National Academy of Sciences of the United States of America
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article