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Novel O-polysaccharide expression, as a lipid A-core-free form, in a lipopolysaccharide-core-defective mutant of Pseudomonas aeruginosa.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Yokota, S. I. |
| Copyright Year | 1996 |
| Abstract | Pseudomonas aeruginosa PML14e is a mutant strain, isolated from strain PML14 (Homma serotype I), that is resistant to all types of R-pyocins. PML14e completely lacked glucose and rhamnose as components of the lipopolysaccharide (LPS) outer core region. Whereas the O-polysaccharide attachment site on the LPS core was considered to be absent, PML14e was agglutinable with anti-serotype-I antibodies. The O-polysaccharide of PML14e was recovered in the supernatant after ultracentrifugation of the aqueous layer from a hot phenol/water extraction. Chromatographic behaviour and chemical analysis indicated that the PML14e O-polysaccharide was not linked to the lipid A. 1H-NMR spectroscopy indicated that the structure of the PML14e O-polysaccharide was the same as that of the O-polysaccharide from PML14. The above evidence indicated that the O-polysaccharide is expressed on the cell surface of the mutant strain PML14e as the lipid A-free form. To examine the nature of the cell surface, the accessibility of monoclonal antibodies (mAbs) against cell surface antigens was tested by enzyme-linked immunosorbent assay. An anti-lipid A mAb and an anti-outer-membrane protein mAb, the epitopes for which are considered to be exposed on rough strains, bound to a greater extent to the PML14e cells than to two other LPS-core-defective rough mutants, PML14b and PML14d. Whereas these mutants appeared to have lesser defects in the LPS core, they expressed less O-polysaccharide than PML14e. The results indicated that the epitopes exposed on rough strains, such as lipid A and outer-membrane proteins, were mainly hindered by covalently linked core oligosaccharide rather than by the O-polysaccharide chain. |
| File Format | PDF HTM / HTML |
| DOI | 10.1099/13500872-142-2-289 |
| Alternate Webpage(s) | http://www.microbiologyresearch.org/docserver/fulltext/micro/142/2/mic-142-2-289.pdf?accname=guest&checksum=8BD65E7954AAEF8DE0103E411C7B2215&expires=1544614857&id=id |
| Alternate Webpage(s) | http://www.microbiologyresearch.org/docserver/fulltext/micro/142/2/mic-142-2-289.pdf?accname=guest&checksum=3B21835421445315A126E1A480EFDFC5&expires=1543528772&id=id |
| PubMed reference number | 8932702 |
| Alternate Webpage(s) | https://doi.org/10.1099/13500872-142-2-289 |
| Journal | Medline |
| Volume Number | 142 |
| Part | 2 |
| Journal | Microbiology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
