Up-regulation of glutamate receptors in nucleus tractus solitarii underlies potentiation of baroreceptor reflex by heat shock protein 70.

Content Provider	Semantic Scholar
Author	Chang, Kui-Fen Ou, Chen-Chun
Copyright Year	2002
Abstract	Whereas induction of the 70-kDa heat shock protein (HSP70) in the nucleus tractus solitarii (NTS), the terminal site in the brain stem for primary baroreceptor afferents, augments baroreceptor reflex (BRR) response, the underlying cellular and molecular mechanism is essentially unexplored. In Sprague-Dawley rats, we evaluated the hypothesis that HSP70 may potentiate BRR response by up-regulating the molecular synthesis and functional expression of glutamate receptors in the NTS. Animals subjected to brief hyperthermic heat shock (HS; 42 degrees C for 15 min) exhibited augmented expression of NR1 or NR2A subunit of N-methyl-D-aspartate (NMDA) receptors, GluR1 or GluR4 subunits of alpha-amino-3-hydroxy-5-methylisoxazole-4-propionate receptors and KA1 subunit of kainate receptors in the NTS. Intriguingly, this up-regulation of glutamate receptors was preceded by an increase in HSP70 expression at the NTS. The HS-induced augmentation in responsiveness of barosensitive NTS neurons to transient hypertension or potentiation of BRR response was discernibly blunted by MK-801 or 6-cyano-7-nitroquinoxaline-2,3-dione. Bilateral microinjection into the NTS of an antisense hsp70 oligonucleotide (50 pmol) before HS significantly suppressed the induced expression of HSP70 or the increase in glutamate receptor subunits in the dorsal medulla and discernibly attenuated the potentiation of BRR response. Control microinjection into the NTS of sense or scrambled hsp70 oligonucleotide (50 pmol) was ineffective. These findings suggest that HSP70 induced by HS may enhance BRR response by up-regulating the molecular synthesis and functional expression of NR1 or NR2A subunit of NMDA receptors and GluR1, GluR4, or KA1 subunit of non-NMDA receptors in the NTS.
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Alternate Webpage(s)	http://molpharm.aspetjournals.org/content/molpharm/61/5/1097.full.pdf
PubMed reference number	11961127v1
Volume Number	61
Issue Number	5
Journal	Molecular pharmacology
Language	English
Access Restriction	Open
Subject Keyword	Aspartic Acid Baroreflex Brain Stem Cell Nucleus Dizocilpine Maleate Fever Glutamate Receptor Glutamic Acid Heat shock proteins Heat-Shock Proteins 70 Heat-Shock Response Hypertensive disease Kainate Microinjections N-Methyl-D-Aspartate Receptors N-Methylaspartate NTS gene Nucleus solitarius Pressoreceptors Receptors, Kainic Acid Reflex action Up-Regulation (Physiology) antisense therapy chemosensitization/potentiation picomole (pM) type I interferon receptor
Content Type	Text
Resource Type	Article