NDLI: Purification and properties of an amylopullulanase, a glucoamylase, and an alpha-glucosidase in the amylolytic enzyme system of Thermoanaerobacterium thermosaccharolyticum.

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Purification and properties of an amylopullulanase, a glucoamylase, and an alpha-glucosidase in the amylolytic enzyme system of Thermoanaerobacterium thermosaccharolyticum.

Content Provider	Semantic Scholar
Author	Ganghofner, D. Kellermann, Joseph Bronnenmeier, Karin
Copyright Year	1998
Abstract	Thermoanaerobic bacteria are of considerable interest as producers of thermostable amylolytic enzymes. The soluble amylolytic enzyme system of Thermoanaerobacterium thermosaccharolyticum DSM 571 was fractionated into a pullulanase, a glucoamylase, and an alpha-glucosidase. The enzymes were purified to homogeneity and their physical and catalytic properties were studied. The pullulanase, which cleaved both alpha-1,4- and alpha-1,6-glucosidic bonds, was an amylopullulanase closely related to similar enzymes from other thermoanaerobic bacteria. Partial amino acid sequences of the glucoamylase were identical with the corresponding sequences deduced from the cga gene encoding the glucoamylase from Clostridium sp. strain G0005. The alpha-glucosidase was identified as an isomaltase belonging to a group of structurally related exo-alpha-1,4-glucosidases and oligo-1,6-glucosidases from bacilli. Comparison of enzyme activities indicated that the glucoamylase had the major amylolytic activity of T. thermosaccharolyticum, with amylopullulanase and alpha-glucosidase assisting in the cleavage of alpha-1,6-glucosidic bonds.
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://ci.nii.ac.jp/els/contents110002678967.pdf?id=ART0002951418
PubMed reference number	9532787v1
Volume Number	62
Issue Number	2
Journal	Bioscience, biotechnology, and biochemistry
Language	English
Access Restriction	Open
Subject Keyword	Alpha-glucosidase Amino Acid Sequence Amino Acids Bacillus Bacteria CHGA wt Allele Cleaved cell Clostridium GLUCOSIDASE Glucan 1,4-alpha-Glucosidase Oligo-1,6-Glucosidase Thermoanaerobacterium amylopullulanase glucan exo-1,3-beta-glucosidase activity pullulanase
Content Type	Text
Resource Type	Article

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