NDLI: Inhibitory effect of leflunomide on hepatic fibrosis induced by CCl4 in rats.

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Inhibitory effect of leflunomide on hepatic fibrosis induced by CCl4 in rats.

Content Provider	Semantic Scholar
Author	Chen, Ji-Qiang Xu, Shu-Yun
Copyright Year	2004
Abstract	AIM To study the effect of leflunomide on CCl4-induced hepatic fibrosis in rats. METHODS Hepatic fibrosis was induced by subcutaneous injection with 50% CCl4 in Sprague-Dawley rats. The amount of CCl4 administered was 1 mg/kg. The alanine aminotransferase (ALT), aspartate aminotransferase (AST), nitric oxide (NO) levels in plasma and hydroxyproline (Hyp) contents in liver tissue were assayed by spectrophotometry. The hyaluronic acid (HA) and procollagen III (PC III) were assessed by radioimmunoassay. The transforming growth factor-beta1 (TGF-beta1) in serum was determined by ELISA. The nuclear factor-kappa B (NF-kappaB) in liver tissue was examined by immunohistochemistry. Liver samples collected after 12 weeks of CCl4 treatment were stained with hematoxylin and eosin. RESULTS Leflunomide (1, 3, and 9 mg/kg) significantly decreased indices of liver and spleen, the serum transaminase (AST, ALT) activities, HA and PC III levels, and Hyp contents in liver tissue in rats of hepatic fibrosis. Histopathological examination showed leflunomide had inhibitory effect on fibrogenesis and formation of pseudolobulus. Furthermore, leflunomide significantly inhibited NF-kappaB expression in liver tissue, and reduced elevated serum TGF-kappa1 and NO levels in rats of hepatic fibrosis. CONCLUSION Leflunomide showed inhibitory action on hepatic fibrosis induced by CCl4 in rats.
File Format	PDF HTM / HTML
PubMed reference number	15210065
Journal	Medline
Volume Number	25
Issue Number	7
Alternate Webpage(s)	http://www.chinaphar.com/article/download/8291/8856
Journal	Acta pharmacologica Sinica
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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