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A new strategy for full-length Ebola virus glycoprotein expression in E.coli
| Content Provider | Semantic Scholar |
|---|---|
| Author | Zai, Junjie Yi, Yinhua Zhang, Bo Yuan, Zhiming |
| Copyright Year | 2016 |
| Abstract | Ebola virus (EBOV) causes severe hemorrhagic fever in humans and non-human primates with high rates of fatality. Glycoprotein (GP) is the only envelope protein of EBOV, which may play a critical role in virus attachment and entry as well as stimulating host protective immune responses. However, the lack of expression of full-length GP in Escherichia coli hinders the further study of its function in viral pathogenesis. In this study, the vp40 gene was fused to the full-length gp gene and cloned into a prokaryotic expression vector. We showed that the VP40-GP and GP-VP40 fusion proteins could be expressed in E.coli at 16 °C. In addition, it was shown that the position of vp40 in the fusion proteins affected the yields of the fusion proteins, with a higher level of production of the fusion protein when vp40 was upstream of gp compared to when it was downstream. The results provide a strategy for the expression of a large quantity of EBOV full-length GP, which is of importance for further analyzing the relationship between the structure and function of GP and developing an antibody for the treatment of EBOV infection. |
| Starting Page | 500 |
| Ending Page | 508 |
| Page Count | 9 |
| File Format | PDF HTM / HTML |
| DOI | 10.1007/s12250-016-3845-y |
| PubMed reference number | 28000060 |
| Journal | Medline |
| Volume Number | 31 |
| Alternate Webpage(s) | https://www.virosin.org/fileZGBDX/journal/article/vs/2016/6/PDF/10.1007s12250-016-3845-y.pdf |
| Alternate Webpage(s) | https://doi.org/10.1007/s12250-016-3845-y |
| Journal | Virologica Sinica |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
