NDLI: Integrated in situ genetic analyzer for microbiology in extreme environments

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Integrated in situ genetic analyzer for microbiology in extreme environments

Content Provider	Semantic Scholar
Author	Fukuba, Tatsuhiro Miyaji, Akimitsu Okamoto, Takuji Yamamoto, Takatoki Kaneda, Shohei Fujii, Teruo
Copyright Year	2011
Abstract	In this study, a totally integrated in situ analyzer for microbial gene detection has been developed for oceanography applications. A PDMS–glass microfluidic device that is capable of cell lysis, DNA purification, PCR, and optical detection has been utilized as the core element of the in situ analyzer. Microbial genomic DNA is purified and concentrated on glass beads packed in the microfluidic device. PCR is performed in a flow-through manner, and the amplified products are fluorescently detected using optical fibers. The sensitivity of a completed analyzer with deep-sea operation capabilities has been evaluated in a laboratory setting, and the analyzer has been operated in real deep-sea environments. Field evaluations have shown that the amplification of the eubacterial universal 16S rRNA gene and the recovery of the PCR product to the surface are successfully achieved in deep-sea environments.
Starting Page	1567
Ending Page	1573
Page Count	7
File Format	PDF HTM / HTML
DOI	10.1039/C1RA00490E
Alternate Webpage(s)	http://www.rsc.org/suppdata/ra/c1/c1ra00490e/c1ra00490e.pdf
Alternate Webpage(s)	https://doi.org/10.1039/C1RA00490E
Volume Number	1
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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