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A case of mosaicism in the germinal line of Odontophrynus americanus (Dùmeril et Bibron. 1841)
| Content Provider | Semantic Scholar |
|---|---|
| Author | Salas, Nancy Edith Martino, Adolfo Ludovico |
| Copyright Year | 2010 |
| Abstract | The objective of this work was to report the presence of cells of different ploidy in a population of Odontophrynus americanus. The cytogenetic study was carried out in three males (ALM 249-251) of the Odontophrynus species captured at Merlo village , San Luis province, Argentina (32° 21 ́S, 65° 02 ́W). The village is located in the western side of the Sierra de Comechingones at 850 meters above sea level. Mitotic metaphases were obtained from testicles after in vivo colchicine treatment according to conventional protocols. All the techniques employed for the cell preparations, hypotonic treatment, fi xation and staining with Giemsa were applied to all the specimens as described in Schmid (1978). Photographs were obtained by using an Axiophot Microscope (Carl Zeiss) with Cannon Powershot G6 Digital Camera and Zoom Browser EX. The cytogenetic study performed in testicular tissues of the three studied males showed the presence of cells with different ploidy. Two germinal lines were observed: diploid (2n = 22) and tetraploid (4n = 44), with frequencies of 35,1 % for diploid cells and 64.9 % for tetraploid cells (Figure 1a, b, Figure 2a, b and Table I). The cytogenetic studies have special importance in the zoological research because by means of karyotype analysis they contribute to the identifi cation of different entities, facilitate the evaluation of the proximity or relationship degree among certain species and fi nally they contribute with fundamental data to the phylogenetic assessment (Schmid et al., 1985; Zampieri et al., 2004). The occurrence of polyploidy in different anuran families that appear as cryptic species highlights the importance of the cytogenetic study at the population level (Valetti et al., 2009). |
| Starting Page | 71 |
| Ending Page | 72 |
| Page Count | 2 |
| File Format | PDF HTM / HTML |
| Volume Number | 21 |
| Alternate Webpage(s) | http://www.scielo.org.ar/pdf/bag/v21n1/v21n1a08.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
