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Nucleotide sequence evidence for the occurrence of three distinct whitefly-transmitted geminiviruses in cassava.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Robinson, David J. Harrison, Bryan D. |
| Copyright Year | 1993 |
| Abstract | The complete nucleotide sequence of the DNA of Indian cassava mosaic virus (ICMV) and a key part of that of a group B isolate of African cassava mosaic virus from Malawi (ACMV-M) were determined and compared at the nucleotide and encoded amino acid levels with the published sequences of an ACMV group A isolate (ACMV-K) and other whitefly-transmitted gemini-viruses (WTGs). The DNA of ICMV consists of two circular single-stranded molecules, DNA-A [2815 nucleotides (nt)] and DNA-B (2645 nt), which differ substantially in sequence from the genome components of ACMV-K (DNA-A 70%, DNA-B 47% sequence identity) and other WTGs. ICMV DNA-A contains eight open reading frames (ORFs) encoding proteins of > 100 amino acid residues, of which four ORFs (one genome sense, three complementary sense) are comparable to those of other WTGs. DNA-B contains one ORF in each sense, as in other WTGs. None of the putative viral proteins are more similar in amino acid sequence to the proteins of ACMV-K than to those of another WTG. The coat protein of ACMV-M is more like that of tomato yellow leaf curl virus from Sardinia (86% sequence identity) than those of ICMV or ACMV-K. The intergenic regions of ACMV-K, ACMV-M and ICMV DNAs differ in size, and largely in sequence, except for two 30 to 40 nt sequences which are also conserved in other WTGs and can form stem-loop structures. The intergenic region of ICMV DNA contains three copies of a 41 nt sequence, and that of ACMV-M DNA contains an imperfect repeat of a 34 nt sequence which resembles the repeated sequence in ICMV DNA. The differences between ACMV-K, ACMV-M and ICMV are considered great enough to justify their separation as isolates of three distinct WTGs: African cassava mosaic virus, East African cassava mosaic virus and Indian cassava mosaic virus. |
| File Format | PDF HTM / HTML |
| DOI | 10.1099/0022-1317-74-11-2437 |
| Alternate Webpage(s) | http://www.microbiologyresearch.org/docserver/fulltext/jgv/74/11/JV0740112437.pdf?accname=guest&checksum=CAED5FA9631F4FFB6AAEC3D9543CA136&expires=1544075855&id=id |
| Alternate Webpage(s) | http://vir.sgmjournals.org/content/74/11/2437.full.pdf |
| Alternate Webpage(s) | https://www.microbiologyresearch.org/docserver/fulltext/jgv/74/11/JV0740112437.pdf?accname=guest&checksum=9A269D360F23AC10B589794AB60E85B9&expires=1564223262&id=id |
| Alternate Webpage(s) | https://www.microbiologyresearch.org/docserver/fulltext/jgv/74/11/JV0740112437.pdf?accname=guest&checksum=AC581362C2B3A2B4AAD04303AE08D27D&expires=1546770866&id=id |
| PubMed reference number | 8245859 |
| Alternate Webpage(s) | https://doi.org/10.1099/0022-1317-74-11-2437 |
| Journal | Medline |
| Volume Number | 74 |
| Part | 11 |
| Journal | The Journal of general virology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
