In vitro growth and shoot multiplication in Terminalia bellerica roxB. under controlled carbon dioxide environment.

Content Provider	Semantic Scholar
Author	Suthar, Rajul Rathore, Pankaj Singh Purohit, Sunil Dutta
Copyright Year	2009
Abstract	The shoot cultures of Terminalia bellerica Roxb. were grown on Murashige and Skoog's medium containing 1.5 mg 1(-1) BAP (6- benzyl aminopurine), and supplemented with or without sucrose (3%). A range of CO2 concentrations (0.0, 0.6, 10, 40 g(-3)) was provided in small acrylic chambers by using different concentrations and combinations of NaHCO3 (sodium bicarbonate), Na2CO3 (sodium carbonate), KHCO3 (potassium bicarbonate) and K2CO3 (potassium carbonate). To obtain a CO2-free environment, a saturated solution of 10% of KOH (potassium hydroxide) was kept in the chamber. Complete absence of carbon source caused death of shoots within 20 days. Under controlled and enriched CO2, the shoots grew fully photoautotropically on sucrose-free medium. The growth of cultures was better with carbon dioxide (40 g(-3)) than sucrose (3%) in the medium. Maximum number of shoots, number of leaves per cluster, fresh and dry weight and chlorophyll contents were recorded when both sucrose and CO2 (40 g(-3)) were provided to the culture.
File Format	PDF HTM / HTML
PubMed reference number	19405387
Journal	Medline
Volume Number	47
Issue Number	3
Alternate Webpage(s)	http://nopr.niscair.res.in/bitstream/123456789/3296/1/IJEB%2047(3)%20204-209.pdf
Journal	Indian journal of experimental biology
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article