NDLI: A new approach to time-resolved studies of ATP-requiring biological systems; laser flash photolysis of caged ATP.

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A new approach to time-resolved studies of ATP-requiring biological systems; laser flash photolysis of caged ATP.

Content Provider	Semantic Scholar
Author	Kihara, Tetsuroh
Copyright Year	1980
Abstract	2-Nitrobenzyl derivatives have been used for several years as photolabile protecting groups in synthetic organic chemistry. Recently, P3-1-(2-nitro phenylethyladenosine 5'-triphosphate "caged ATP" was synthesized and its photolysis was shown to generate ATP in situ. This and related reactions have great potential for structural and kinetic studies of both intact and soluble biological systems and it is thus important to define the kinetic characteristics of the photolytic reaction. Caged ATP (2.5 mM) was photolyzed at 347 nm by a single 30-nsec pulse from a frequency-doubled ruby laser of 25 mJ energy to generate 500 microM ATP. The kinetics of the overall reaction were determined by monitoring the kinetics of ATP-induced dissociation of actomyosin, a reaction of known kinetic characteristics. Release of 500 microM ATP was found to be controlled by a process having a rate constant of 2.2 X 10(9) [H+] sec-1 at 22 degrees C at pH 5.8-9.5, which corresponds to 220 sec-1 at pH 7. This process is believed to be the breakdown of an aci-nitro compound, which was identified on the basis of its spectral properties and the photochromicity of related 2-nitrobenzyl compounds.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://nathan.instras.com/MyDocsDB/doc-717.pdf
PubMed reference number	6938971v1
Volume Number	77
Issue Number	12
Journal	Proceedings of the National Academy of Sciences of the United States of America
Language	English
Access Restriction	Open
Subject Keyword	Adenosine Triphosphate Catabolism Chemistry, Organic Event-Related Potentials, P300 Flash photolysis Kinetics (discipline) Leukemoid Reaction Nitro Compounds Rats, Inbred ACI actomyosin location
Content Type	Text
Resource Type	Article

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