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Apelin stimulates proliferation and suppresses apoptosis of mouse osteoblastic cell line MC3T3-E1 via JNK and PI3-K/Akt signaling pathways
| Content Provider | Semantic Scholar |
|---|---|
| Author | Tang, Siyuan Yuan, Ling-Qing Luo, Xiang-Hang Liao, Er-Yuan |
| Copyright Year | 2007 |
| Abstract | The aim of this study was to investigate the effects of apelin on proliferation and apoptosis of mouse osteoblastic MC3T3-E1 cells. APJ was expressed in MC3T3-E1 cells. Apelin did not affect Runx2 expression, alkaline phosphatase (ALP) activity, osteocalcin and type I collagen secretion, suggesting that it has no effect on osteoblastic differentiation of MC3T3-E1 cells. However, apelin stimulated MC3T3-E1 cell proliferation and inhibited cell apoptosis induced by serum deprivation. Our study also shows that apelin decreased cytochrome c release and caspase-3, capase-8 and caspase-9 activation in serum-deprived MC3T3-E1 cells. Apelin activated c-Jun N-terminal kinase (JNK) and Akt (phosphatidylinositol 3-kinase downstream effector), and the JNK inhibitor SP600125, the phosphatidylinositol 3-kinase (PI3-K) inhibitor LY294002 or the Akt inhibitor 1L-6-hydroxymethyl-chiro-inositol 2-(R)-2-O-methyl-3-O-octadecylcarbonate (HIMO) inhibited its effects on proliferation and serum deprivation-induced apoptosis. Furthermore, apelin protected against apoptosis induced by the glucocorticoid dexamethasone or TNF-alpha. Apelin stimulates proliferation and suppresses serum deprivation-induced apoptosis of MC3T3-E1 cells and these actions are mediated via JNK and PI3-K/Akt signaling pathways. |
| Starting Page | 708 |
| Ending Page | 718 |
| Page Count | 11 |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/j.peptides.2006.10.005 |
| PubMed reference number | 17109997 |
| Journal | Medline |
| Volume Number | 28 |
| Alternate Webpage(s) | https://api.elsevier.com/content/article/pii/S0196978106004414 |
| Alternate Webpage(s) | https://www.sciencedirect.com/science/article/pii/S0196978106004414?dgcid=api_sd_search-api-endpoint |
| Alternate Webpage(s) | https://doi.org/10.1016/j.peptides.2006.10.005 |
| Journal | Peptides |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
