NDLI: SipA, SopA, SopB, SopD and SopE2 effector proteins of Salmonella enterica serovar Typhimurium are synthesized at late stages of infection in mice.

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SipA, SopA, SopB, SopD and SopE2 effector proteins of Salmonella enterica serovar Typhimurium are synthesized at late stages of infection in mice.

Content Provider	Semantic Scholar
Author	Giacomodonato, Mónica Nancy Uzzau, Sergio Bacciu, Donatella Caccuri, Roberto L. Sarnacki, Sebastián Hernán Rubino, S. Cerquetti, María Cristina
Copyright Year	2007
Abstract	Salmonella pathogenicity island (SPI)-1 is essential for invasion of non-phagocytic cells, whereas SPI-2 is required for intracellular survival and proliferation in phagocytes. Some SPI-1 effectors, however, are induced upon invasion of both phagocytic and non-phagocytic cells, suggesting that they may also be required post-invasion. In the present work, the presence was analysed of SipA, SopA, SopB, SopD and SopE2 effector proteins of Salmonella enterica serovar Typhimurium in vitro and in vivo during murine salmonellosis. Tagged (3xFLAG) strains of S. enterica serovar Typhimurium were inoculated intraperitoneally or intragastrically to BALB/c mice and recovered from the spleen and mesenteric lymph nodes of moribund mice. Tagged proteins were detected by SDS-PAGE and immunoblotting with anti-FLAG antibodies. In vitro experiments showed that SPI-1 effector proteins SipA, SopA, SopB, SopD and SopE2 were secreted under SPI-1 conditions. Interestingly, it was found that S. enterica serovar Typhimurium continued to synthesize SipA, SopB, SopD and SopE2 in colonized organs for several days, regardless of the route of inoculation. Together, these results indicate that SPI-1 effector proteins may participate in the late stages of Salmonella infection in mice.
File Format	PDF HTM / HTML
DOI	10.1099/mic.0.2006/002758-0
PubMed reference number	17379731
Journal	Medline
Volume Number	153
Part	4
Alternate Webpage(s)	https://www.microbiologyresearch.org/docserver/fulltext/micro/153/4/1221.pdf?accname=guest&checksum=602C16942F12CFE462A6C5399264EC64&expires=1558766638&id=id
Alternate Webpage(s)	http://www.microbiologyresearch.org/docserver/fulltext/micro/153/4/1221.pdf?accname=guest&checksum=BDFEF521800EB0263E77CD328791A11B&expires=1544010096&id=id
Alternate Webpage(s)	https://doi.org/10.1099/mic.0.2006%2F002758-0
Journal	Microbiology
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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