Salinarimonas ramus sp. nov. and Tessaracoccus oleiagri sp. nov., isolated from a crude oil-contaminated saline soil.

Content Provider	Semantic Scholar
Author	Wang, Lu Wang, Ya-Nan Tang, Yue-Qin Wu, Xiao-Lei
Copyright Year	2011
Abstract	Four bacterial strains, SL014B-41A4(T), SL014B-20A1(T), SL014B-76A1 and SL014B-79A, isolated from a crude oil-contaminated saline soil of Shengli Oilfield, China, were investigated using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SL014B-41A4(T) belonged to the genus Salinarimonas in the order Rhizobiales, with the highest sequence similarity with Salinarimonas rosea YIM YD3(T) (98.3 %). The DNA-DNA relatedness of strain SL014B-41A4(T) to S. rosea YIM YD3(T) was 27.03 ± 3.0 %. Strain SL014B-41A4(T) was Gram-negative staining, facultatively anaerobic and produced deep red pigment in artificial seawater medium. Cells of strain SL014B-41A4(T) were rod-shaped (0.6-4.0 × 1.25-25 µm), motile with a single polar flagellum and often formed branches. The strain contained Q-10 as the predominant respiratory ubiquinone and C(18 : 1)ω7c (57.5 %), C(16 : 0) (16.4 %) and 10-methyl C(19 : 0) (9.1 %) as the major fatty acids. Strains SL014B-20A1(T), SL014B-76A1 and SL014B-79A were actinobacteria and belonged to the genus Tessaracoccus in the family Propionibacteriaceae of the order Actinomycetales with the highest 16S rRNA gene sequence similarities with Tessaracoccus flavescens SST-39(T) (96.4 %), Tessaracoccus lubricantis KISS-17Se(T) (96.2 %) and Tessaracoccus bendigoensis Ben 106(T) (94.7 %). Strains SL014B-20A1(T), SL014B-76A1 and SL014B-79A were Gram-positive staining, facultatively anaerobic, non-endospore-forming, non-motile, acid-fast and oval to rod-shaped (0.48 × 0.5-1.0 µm). These three novel strains had ll-diaminopimelic acid (DAP) as the diagnostic diamino acid in the cell-wall peptidoglycan, MK-9(H(4)) as the only menaquinone and anteiso-C(15 : 0) (67.11-76.14 %) as the major cellular fatty acid. The G+C contents of the genomic DNA of strain SL014B-41A4(T) and strains SL014B-20A1(T), SL014B-76A1 and SL014B-79A were 67.68 mol% and 65.65-67.17 mol%, respectively. Based on phenotypic and genotypic characteristics, strain SL014B-41A4(T) represents a novel species of the genus Salinarimonas, for which the name Salinarimonas ramus is proposed, with strain SL014B-41A4(T) ( = DSM 22962(T) = CGMCC 1.9161(T)) as the type strain. Strains SL014B-20A1(T), SL014B-76A1 and SL014B-79A represent a novel species of the genus Tessaracoccus, for which the name Tessaracoccus oleiagri is proposed, with strain SL014B-20A1(T) ( = DSM 22955(T) = CGMCC 1.9159(T)) as the type strain.
File Format	PDF HTM / HTML
DOI	10.1099/ijs.0.025932-0
PubMed reference number	20802058
Journal	Medline
Volume Number	61
Part	8
Alternate Webpage(s)	https://www.microbiologyresearch.org/docserver/fulltext/ijsem/61/8/1767_ijs025932.pdf?accname=guest&checksum=279D977B9E9F6BDEB647F5C9F824BDFE&expires=1552175771&id=id
Alternate Webpage(s)	https://www.microbiologyresearch.org/docserver/fulltext/ijsem/61/8/1767_ijs025932.pdf?accname=guest&checksum=464312007F4876309453672ACBD93DB3&expires=1545437702&id=id
Alternate Webpage(s)	http://www.microbiologyresearch.org/docserver/fulltext/ijsem/61/8/1767_ijs025932.pdf?accname=guest&checksum=0D4A3CB24AB75B218E8AB932D1FF9E55&expires=1541313376&id=id
Alternate Webpage(s)	https://www.microbiologyresearch.org/docserver/fulltext/ijsem/61/8/1767_ijs025932.pdf?accname=guest&checksum=B27DB2987EA547A91150F5E96CE6C5E6&expires=1547073272&id=id
Alternate Webpage(s)	https://www.microbiologyresearch.org/docserver/fulltext/ijsem/61/8/1767_ijs025932.pdf?accname=guest&checksum=11C162DC391063BF57489D16D397B75A&expires=1557891526&id=id
Journal	International journal of systematic and evolutionary microbiology
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article