The impact of probiotic Lactobacillus casei and Lactobacillus paracasei on zonula occludens 1 production in enterocytes of gnotobiotic mice

Content Provider	Semantic Scholar
Author	Rosiak, Ilona Brudek, Magda Kozakova, Hana
Copyright Year	2009
Abstract	Intestinal permeability plays a role in pathogenesis of several disorders, in which probiotics are successfully used. The regulation of intestinal barrier integrity is dependent on zonula occludens (ZO) proteins which link cytoskeleton actin filaments and intercellular tight junctions of epithelial cells. The aim of the study was to evaluate the influence of the probiotic mixture of Lactobacillus casei LOCK 0900, Lactobacillus casei LOCK 0908 and Lactrobacillus paracasei LOCK 0919 on expression of ZO-1 protein in ileum of gnotobiotic mice. ZO-1 was visualized by the immunohistochemical technique and quantitatively analyzed by morphometric Cell P program in germ-free (GF) mice associated with the mixture of probiotics, GF mice and mice reared in conventional conditions (CONV). The largest amounts of ZO-1 were found in epithelial cells of CONV mice (mean 41,58%), and the quantity of this protein was significantly higher in comparison with GF mice (mean 13,54%). Association of GF animals with Lactobacillus strains induced significantly higher synthesis of ZO-1 in intestinal epithelium in comparison with GF mice (27,4% versus 13,54%). We conclude that probiotic Lactobacillus casei and Lactobacillus paracasei strains, which were shown to induce clinical improvement in children with cow’s milk protein allergy could exhibit beneficial effects by modulation of the intestinal barrier function.
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Language	English
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