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Antibody-dependent cell-mediated cytotoxicity in simian immunodeficiency virus-infected rhesus monkeys.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Sun, Yue Asmal, Mohammed Lane, Sophie Permar, Sallie R. Schmidt, Stephen D. Mascola, John R. Letvin, Norman L. |
| Copyright Year | 2011 |
| Abstract | With the recent demonstration in the RV144 Thai trial that a vaccine regimen that does not elicit neutralizing antibodies or cytotoxic T lymphocytes may confer protection against human immunodeficiency virus type 1 (HIV-1) infection, attention has turned to nonneutralizing antibodies as a possible mechanism of vaccine protection. In the current study, we evaluated the kinetics of the antibody-dependent cell-mediated cytotoxicity (ADCC) response during acute and chronic SIVmac251 infection of rhesus monkeys. We first adapted a flow cytometry-based ADCC assay, evaluating the use of different target cells as well as different strategies for quantitation of activated natural killer (NK) cells. We found that the use of SIVmac251 Env gp130-coated target cells facilitates analyses of ADCC activity with a higher degree of sensitivity than the use of simian immunodeficiency virus (SIV)-infected target cells; however, the kinetics of the measured responses were the same using these different target cells. By comparing NK cell expression of CD107a with NK cell expression of other cytokines or chemokine molecules, we found that measuring CD107a expression is sufficient for evaluating the anti-SIV function of NK cells. We also showed that ADCC responses can be detected as early as 3 weeks after SIVmac251 infection and that the magnitude of this antibody response is inversely associated with plasma viral RNA levels in animals with moderate to high levels of viral replication. However, we also demonstrated an association between NK cell-mediated ADCC responses and the amount of SIVmac251 gp140 binding antibody that developed after viral infection. This final observation raises the possibility that the antibodies that mediate ADCC are a subset of the antibodies detected in a binding assay and arise within weeks of infection. |
| Starting Page | 265 |
| Ending Page | 269 |
| Page Count | 5 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://jvi.asm.org/content/85/14/6906.full.pdf |
| PubMed reference number | 21593181v1 |
| Alternate Webpage(s) | https://doi.org/10.1128/JVI.00326-11 |
| DOI | 10.1128/jvi.00326-11 |
| Journal | Journal of virology |
| Volume Number | 85 |
| Issue Number | 14 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Antibody Formation Antibody-Dependent Cellular Cytotoxicity Cell-Mediated Cytolysis Flow Cytometry HIV Antibodies HIV Infections HIV-1 Helper-Inducer T-Lymphocyte Immunologic Deficiency Syndromes Infection Leukemia, B-Cell Macaca mulatta Monkeys Natural Killer Cells Quantitation RNA Rh Isoimmunization Simian immunodeficiency virus Subgroup Virus Diseases Virus Replication autologous Epstein-Barr virus-specific cytotoxic T lymphocytes (cell) viral capsid secondary envelopment |
| Content Type | Text |
| Resource Type | Article |
