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The 193-Kd Vault Protein, Vparp, Is a Novel Poly(Adp-Ribose) Polymerase
| Content Provider | Semantic Scholar |
|---|---|
| Author | Kickhoefer, Valerie A. Siva, Amara C. Kedersha, Nancy L. Inman, Elisabeth M. Ruland, Charles M. Streuli, Michel Rome, Leonard H. |
| Copyright Year | 1999 |
| Abstract | Mammalian vaults are ribonucleoprotein (RNP) complexes, composed of a small ribonucleic acid and three proteins of 100, 193, and 240 kD in size. The 100-kD major vault protein (MVP) accounts for >70% of the particle mass. We have identified the 193-kD vault protein by its interaction with the MVP in a yeast two-hybrid screen and confirmed its identity by peptide sequence analysis. Analysis of the protein sequence revealed a region of approximately 350 amino acids that shares 28% identity with the catalytic domain of poly(ADP-ribose) polymerase (PARP). PARP is a nuclear protein that catalyzes the formation of ADP-ribose polymers in response to DNA damage. The catalytic domain of p193 was expressed and purified from bacterial extracts. Like PARP, this domain is capable of catalyzing a poly(ADP-ribosyl)ation reaction; thus, the 193-kD protein is a new PARP. Purified vaults also contain the poly(ADP-ribosyl)ation activity, indicating that the assembled particle retains enzymatic activity. Furthermore, we show that one substrate for this vault-associated PARP activity is the MVP. Immunofluorescence and biochemical data reveal that p193 protein is not entirely associated with the vault particle, suggesting that it may interact with other protein(s). A portion of p193 is nuclear and localizes to the mitotic spindle. |
| Starting Page | 917 |
| Ending Page | 928 |
| Page Count | 12 |
| File Format | PDF HTM / HTML |
| PubMed reference number | 10477748 |
| Volume Number | 146 |
| Journal | The Journal of cell biology |
| Alternate Webpage(s) | http://jcb.rupress.org/content/146/5/917.full.pdf |
| Alternate Webpage(s) | http://ftp.ncbi.nlm.nih.gov/pub/pmc/8b/d9/9906061.PMC2169495.pdf |
| Alternate Webpage(s) | http://jcb.rupress.org/content/jcb/146/5/917.full.pdf |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Adenosine Diphosphate Amino Acid Sequence Amino Acids DNA Damage MVP gene Mitotic Spindle Apparatus NAD+ ADP-ribosyltransferase activity Poly(ADP-ribose) Polymerases RNA Ribonucleoproteins Ribose Sequence Analysis Sequence Analysis, Protein |
| Content Type | Text |
| Resource Type | Article |
