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Plant regeneration from embryogenic suspension cultures of Musa acuminata cv. Mas (AA)
| Content Provider | Semantic Scholar |
|---|---|
| Author | Jalil, Mahanom Khalid, Norzulaani Othman, Rofina Yasmin |
| Copyright Year | 2004 |
| Abstract | Embryogenic callus was established using immature male flower of Musa acuminata cv. Mas. After 5–6 months of culture, embryogenic callus was obtained at 21.75±11.9 from 750 immature male flower clusters with translucent somatic embryos proliferated from the whitish friable callus. It was observed that flower clusters ranging from 4 to 11 responded to form embryogenic callus and out of which 3–10 somatic embryos were formed per flower cluster. Embryogenic callus were obtained at a percentage of 10.00±0.3 on M1 medium initially supplemented with 18 μM 2,4-dichlorophenoxyacetic acid (2,4-D) for 3 months and subsequently transferred to the same media with reduced 2,4-D (9 μM) for the next 2–3 months. Embryos developed into translucent spheres and slightly torpedo shaped embryos in suspension cultures. Plantlets were obtained on medium M4 supplemented with 0.8μM BA, at an average regeneration rate of 13.00±0.58. |
| Starting Page | 209 |
| Ending Page | 214 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| DOI | 10.1023/A:1025814922547 |
| Alternate Webpage(s) | https://umexpert.um.edu.my/file/publication/00004390_6335_75881.pdf |
| Alternate Webpage(s) | https://doi.org/10.1023/A%3A1025814922547 |
| Volume Number | 75 |
| Journal | Plant Cell, Tissue and Organ Culture |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
