NDLI: Estabelecimento da técnica de citopatologia no laboratório de patologia oral da Faculdade de Odontologia da Universidade Federal Fluminense – Instituto de Saúde de Nova Friburgo

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Estabelecimento da técnica de citopatologia no laboratório de patologia oral da Faculdade de Odontologia da Universidade Federal Fluminense – Instituto de Saúde de Nova Friburgo

Content Provider	Semantic Scholar
Author	Reys, Irma Gonçalves Silva, Hêmilly Da
Copyright Year	2016
Abstract	Microorganisms play a critical role in the etiology and pathogenesis of apical periodontitis. Enterococcus faecalis and Candida are frequently associated with persistent infections. The aim of this study was to evaluate the antimicrobial effects of primary endodontic conventional treatment, followed by an adjunct therapy with the antimicrobial photodynamic therapy (aPDT). Microbial detection was performed by polymerase chain reaction (PCR). Ten uniradicular teeth [control group (CG)= 4 and test group (TG)= 6] with endodontic infections were analyzed. Samples were collected before and after chemical-mechanical instrumentation (CMI), after the aPDT (for the TG) and after removed the temporary obturation at the second session. In TG the aPDT was performed with low intensity laser diode (methylene blue/ 5 min; 1J/cm; λ = 660 nm; 100 mW; 40 s). The genes for E. faecalis, Candida sp. and Bacteria domain were detected. For TG, the tooth that was positive for Candida before CMI was not detected in subsequent samples, additionally E. faecalis was not detected at the second session, but the species was present in two teeth after CMI and one after aPDT. The aPDT may be used as an effective adjunct, resulting in a significant reduction of the incidence of E. faecalis before final obturation.
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://app.uff.br/riuff/bitstream/1/10748/1/Rayane%20Paula%20Machado%20da%20Silva.pdf
Alternate Webpage(s)	https://app.uff.br/riuff/bitstream/1/12770/1/Disserta%C3%A7%C3%A3o%20final%20Carol.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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