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Expression of connexins in human preimplantation embryos in vitro
| Content Provider | Semantic Scholar |
|---|---|
| Author | Bloor, Debra J. Wilson, Yvonne Kibschull, Mark Traub, Otto Leese, Henry J. Winterhager, Elke Kimber, Susan J. |
| Copyright Year | 2004 |
| Abstract | Intercellular communication via gap junctions is required to coordinate developmental processes in the mammalian embryo. We have investigated if the connexin (Cx) isoforms known to form gap junctions in rodent preimplantation embryos are also expressed in human embryos, with the aim of identifying species differences in communication patterns in early development. Using a combination of polyA PCR and immunocytochemistry we have assessed the expression of Cx26, Cx31, Cx32, Cx40, Cx43 and Cx45 which are thought to be important in early rodent embryos. The results demonstrate that Cx31 and Cx43 are the main connexin isoforms expressed in human preimplantation embryos and that these isoforms are co-expressed in the blastocyst. Cx45 protein is expressed in the blastocyst but the protein may be translated from a generally low level of transcripts: which could only be detected in the PN to 4-cell embryos. Interestingly, Cx40, which is expressed by the extravillous trophoblast in the early human placenta, was not found to be expressed in the blastocyst trophectoderm from which this tissue develops. All of the connexin isoforms in human preimplantation embryos are also found in rodents pointing to a common regulation of these connexins in development of rodent and human early embryos and perhaps other species. |
| Starting Page | 25 |
| Ending Page | 25 |
| Page Count | 1 |
| File Format | PDF HTM / HTML |
| PubMed reference number | 15175102 |
| Volume Number | 2 |
| Journal | Reproductive biology and endocrinology : RB&E |
| Alternate Webpage(s) | http://download-redirector.springer.com/redirect?contentType=pdf&ddsId=art:10.1186/1477-7827-2-25&originUrl=http://rbej.biomedcentral.com/article/10.1186/1477-7827-2-25 |
| Alternate Webpage(s) | http://eprints.whiterose.ac.uk/1035/1/bmc_1477-7827-2-25.pdf |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Blastocyst structure Connexins Embryo Embryo, Mammalian GJA5 gene GJB2 protein, human GJB3 gene Gap Junctions Immunocytochemistry Mammals Preimplantation Diagnosis Protein Isoforms Rodent Transcript trophoblast |
| Content Type | Text |
| Resource Type | Article |
