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Crystal structure of a core spliceosomal protein interface.

Content Provider	Semantic Scholar
Author	Schellenberg, Matthew J. Edwards, Ross A. Ritchie, Dustin B. Kent, Oliver A. Golas, Monika Mariola Stark, Holger Lührmann, Reinhard Glover, J. N. Mark Millan, Andrew M. Mac
Copyright Year	2006
Abstract	The precise excision of introns from precursor mRNAs (pre-mRNAs) in eukaryotes is accomplished by the spliceosome, a complex assembly containing five small nuclear ribonucleoprotein (snRNP) particles. Human p14, a component of the spliceosomal U2 and U11/U12 snRNPs, has been shown to associate directly with the pre-mRNA branch adenosine early in spliceosome assembly and within the fully assembled spliceosome. Here we report the 2.5-A crystal structure of a complex containing p14 and a peptide derived from the p14-associated U2 snRNP component SF3b155. p14 contains an RNA recognition motif (RRM), the surface of which is largely occluded by a C-terminal alpha-helix and a portion of the SF3b155 peptide. An analysis of RNA.protein crosslinking to wild-type and mutant p14 shows that the branch adenosine directly interacts with a conserved aromatic within a pocket on the surface of the complex. This result, combined with a comparison of the structure with known RRMs and pseudoRRMs as well as model-building by using the electron cryomicroscopy structure of a spliceosomal U11/U12 di-snRNP, suggests that p14.SF3b155 presents a noncanonical surface for RNA recognition at the heart of the mammalian spliceosome.
Starting Page	89
Ending Page	98
Page Count	10
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www2.biochem.ualberta.ca/labs/macmillan/MacMillan27.pdf
Alternate Webpage(s)	http://pubman.mpdl.mpg.de/pubman/item/escidoc:641739:3/component/escidoc:641738/287786.pdf
Alternate Webpage(s)	http://www.genome.ou.edu/5853/splicing/crystalstructure-core-splicesome.pdf
PubMed reference number	16432215v1
Volume Number	103
Issue Number	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Language	English
Access Restriction	Open
Subject Keyword	AROMATICS Cross link Cryoelectron Microscopy Excision Interface Device Component Introns Mammals Protein Domain RNA Recognition Motif Ribonucleoprotein, U2 Small Nuclear Small Nuclear Ribonucleoproteins Spliceosome Assembly Pathway Spliceosomes negative regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
Content Type	Text
Resource Type	Article

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