Multiple immunoglobulin heavy-chain gene transcripts in Abelson murine leukemia virus-transformed lymphoid cell lines.

Content Provider	Semantic Scholar
Author	Alt, Frederick W. Rosenberg, Naomi E. Enea, Vincenzo Siden, Edward J. Baltimore, David
Copyright Year	1982
Abstract	Lymphoid cells transformed by Abelson murine leukemia virus (A-MuLV) contain three classes of RNA transcripts from immunoglobulin mu genes. P mu-mRNAs (productive) correspond to the normal 2.7-kilobase (kb) membrane (mu m) and 2.4-kb secreted (mu s) mu mRNA species both in size and coding capacity and occur at approximately equal abundance in most mu-positive (pre-B-like) A-MuLV transformants. A mu-mRNAs (aberrant) generally fall into one of two categories--aberrantly small 2.3-kb mu m and 2.0-kb mu s mRNAs which encode aberrantly small mu polypeptide chains, or normal-sized, V H-containing mu RNAs which do not encode immunologically identifiable mu polypeptide chains. In one case, the latter type of A mu-mRNA was demonstrated to result from an in-phase termination codon in the D segment of the mu mRNA. Also, most, if not all, A-MuLV transformants express members of a 3.0 to 1.9-kb set of C mu-containing, but V H-negative S mu-RNAs (for sterile), the expression of which may occur simultaneously with but independently of P mu-mRNAs or A mu-mRNAs. The S mu-RNA sequences do not encode immunologically identifiable mu chains and can be produced by cells with unrearranged heavy-chain alleles, such as T-lymphocytes, although the structure of the S mu-RNAs from T-lymphoid cells appears to be different from that of B-lymphoid cell S mu-RNAs. Certain A-MuLV transformants also express gamma-RNA sequences that are probably analogous to the three different forms of mu RNA. These data support the concept that heavy-chain allelic exclusion, like that of light chains, is not mediated by control at the DNA or RNA levels but is probably a consequence of feedback control from cytoplasmic mu chains.
Starting Page	386
Ending Page	400
Page Count	15
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://authors.library.caltech.edu/6960/1/ALTmcb82.pdf
PubMed reference number	6810096v1
Volume Number	2
Issue Number	4
Journal	Molecular and cellular biology
Language	English
Access Restriction	Open
Subject Keyword	Abelson murine leukemia virus Categories Cell secretion Class Codon, Terminator Encode (action) Exclusion Infertility Leukemia, B-Cell Murine sarcoma viruses Polypeptides RNA Tissue membrane Transcript
Content Type	Text
Resource Type	Article