NDLI: Negative Electron Transfer Dissociation Sequencing of 3-O-Sulfation-Containing Heparan Sulfate Oligosaccharides

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Negative Electron Transfer Dissociation Sequencing of 3-O-Sulfation-Containing Heparan Sulfate Oligosaccharides

Content Provider	Semantic Scholar
Author	Wu, Jiandong Wei, Juan Hogan, John D. Chopra, Pradeep Joshi, Apoorva Lu, Weigang Klein, Joshua A. Boons, Geert-Jan Lin, Cheng Zaia, Joseph
Copyright Year	2018
Abstract	AbstractAmong dissociation methods, negative electron transfer dissociation (NETD) has been proven the most useful for glycosaminoglycan (GAG) sequencing because it produces informative fragmentation, a low degree of sulfate losses, high sensitivity, and translatability to multiple instrument types. The challenge, however, is to distinguish positional sulfation. In particular, NETD has been reported to fail to differentiate 4-O- versus 6-O-sulfation in chondroitin sulfate decasaccharide. This raised the concern of whether NETD is able to differentiate the rare 3-O-sulfation from predominant 6-O-sulfation in heparan sulfate (HS) oligosaccharides. Here, we report that NETD generates highly informative spectra that differentiate sites of O-sulfation on glucosamine residues, enabling structural characterizations of synthetic HS isomers containing 3-O-sulfation. Further, lyase-resistant 3-O-sulfated tetrasaccharides from natural sources were successfully sequenced. Notably, for all of the oligosaccharides in this study, the successful sequencing is based on NETD tandem mass spectra of commonly observed deprotonated precursor ions without derivatization or metal cation adduction, simplifying the experimental workflow and data interpretation. These results demonstrate the potential of NETD as a sensitive analytical tool for detailed, high-throughput structural analysis of highly sulfated GAGs. Graphical Abstract
Starting Page	1262
Ending Page	1272
Page Count	11
File Format	PDF HTM / HTML
DOI	10.1007/s13361-018-1907-0
PubMed reference number	29564812
Journal	Medline
Volume Number	29
Alternate Webpage(s)	https://rd.springer.com/content/pdf/10.1007/s13361-018-1907-0.pdf
Alternate Webpage(s)	https://doi.org/10.1007/s13361-018-1907-0
Journal	Journal of The American Society for Mass Spectrometry
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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