Synthesis and use of disulfide-bas ed H-phosphonate reagent for 3'- and/or 5'- oligonucleotide labelling via mercaptoalkyl linker

Content Provider	Semantic Scholar
Author	Dubey, I. Ya. Fedoryak, D. M.
Copyright Year	1998
Abstract	Synthesis of new disulfide-based H-phosphonate reagent for solid-phase oligonucleotide functionalization with mercaptopropyl group at one or two termini is described. Using this single reagent, 3'- and 3', 5'-disulfide-linked oligonucleotides were synthesized', disulfide bonds cleaved with dithiothreitol and generated thiol groups labelled with iodoacetamidofluorescein. Tis oligonucleotides containing one or two fluorescein residues at 3'- and 5'-ends were prepared in high yields.
Starting Page	163
Ending Page	172
Page Count	10
File Format	PDF HTM / HTML
DOI	10.7124/bc.0004CA
Volume Number	14
Alternate Webpage(s)	http://biopolymers.org.ua/pdf/14/2/163/biopolym.cell-1998-14-2-163-en.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article