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Protein recognition by bivalent, ‘turn-on’ fluorescent molecular probes† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c5sc01038a Click here for additional data file.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Unger-Angel, Linor Rout, Bhimsen Ilani, Tal Eisenstein, Miriam Motiei, Leila Margulies, David |
| Copyright Year | 2015 |
| Abstract | We show that the conversion of a known intercalating dye (i.e., thiazole orange) into a bivalent protein binder could lead to the realization of a novel class of 'turn-on' fluorescent molecular probes that detect proteins with high affinity, selectivity, and a high signal-to-noise (S/N) ratio. The feasibility of the approach is demonstrated with monomolecular probes that light-up in the presence of three different proteins: acetylcholinesterase (AChE), glutathione-s-transferase (GST), or avidin (Av) at low concentrations and with minimal background signal. The way by which such probes can be used to detect individual protein isoforms and be applied in inhibitor screening, cell imaging, and biomarker detection is described. |
| Starting Page | 5419 |
| Ending Page | 5425 |
| Page Count | 7 |
| File Format | PDF HTM / HTML |
| DOI | 10.1039/c5sc01038a |
| PubMed reference number | 28717444 |
| Journal | Medline |
| Volume Number | 6 |
| Alternate Webpage(s) | http://www.rsc.org/suppdata/c5/sc/c5sc01038a/c5sc01038a1.pdf |
| Alternate Webpage(s) | https://pubs.rsc.org/en/content/articlepdf/2015/sc/c5sc01038a |
| Alternate Webpage(s) | http://www.weizmann.ac.il/Organic_Chemistry/Margulies/sites/Organic_Chemistry.Margulies/files/pub/13_0.pdf |
| Alternate Webpage(s) | https://doi.org/10.1039/c5sc01038a |
| Journal | Chemical science |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
