Dopam ine — induced ionic currents in acutely dissociated rat neurons of CNS

Content Provider	Semantic Scholar
Copyright Year	2004
Abstract	KEY W ORDS dopamine;potassium channels; DA induced response. CONCLUSION : These hippocampus pyramidal cells;patch clamp tech— results suggest that DA —induced outward current niques is carried by K in single hippocampal CA1 pyra— mjdal neuron. AIM :To determine whether or not the dopamine (DA )can induce ionic current in single neuron a— cutely dissociated from different centra1 areas in— eluding striatum ,ventral tegmental area(VTA ), substantia nigra pars compacta (SNC) and hip— pocampal CA1 area. M ETHODS:Using a new patch—clamp whole—cell recording technic,namely nystatin—perforated whole—cell configuration un—r der voltage—clamp mode. RESULTS#In 36 sin— gle neurons isolated from the striatum and VTA . DA 1O— l000“mo卜LI1 was quite diverse to elicit ionic current response. In 19 SNC neurons, 5 neurons(26 )response to 1 mmo卜L-1 DA rep— resented as a small outward current(11.3土 2.4 pA)at a holding potential(VH)of一 20 mV. In 25 0f 69 (36 )examined hippocampal CA1 pyra— mida1 neurons, however,application of DA in— duced 3 types of current responses:outward cur— rent(8 neurons)accompanied with an increase of mem brane c0nductance.slow inward current (5 neurons)with an decrease of mem brane conduc— tance and outward—following inward current (1 2 neurons)at a VH of 20 mV. The concentra— tion—response relationship of DA—induced currents showed the typical sigmoid shape with the threshold dose,being the maxium response dose are 3 tLmoI·L and 1 mmoI'L respectively. The current—voltage (I—V ) relationship of DA— induced responses did not show any voltagedependent manner and the reversa1 potential (EM )was close to the equilibrium potentia1 of potassium (EK)calculated with the Nernst equa tion. TEA 5 mo1·L effectively inhibited the
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