NDLI: Transcription mapping of human herpesvirus 8 genes encoding viral interferon regulatory factors.

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Transcription mapping of human herpesvirus 8 genes encoding viral interferon regulatory factors.

Content Provider	Semantic Scholar
Author	Cunningham, Charles Barnard, Suzanne Blackbourn, David J. Davison, Andrew J.
Copyright Year	2003
Abstract	The human herpesvirus 8 (HHV-8) genome contains four tandemly arranged genes encoding viral interferon regulatory factors (vIRF-1 to 4) located between genes 57 and 58. Transcript mapping techniques were employed to determine the sizes, ends and splicing patterns of mRNAs specified by these genes in HHV-8-infected cell lines untreated or chemically induced into the lytic growth cycle. Depending on the cell line used, vIRF-3 transcription was minimally or not induced (i.e. expressed with latent kinetics), whereas the other vIRFs were inducible (i.e. expressed with lytic kinetics). Each gene possessed its own promoter (or promoters) and polyadenylation sites, and all but vIRF-1 were spliced from two exons. vIRF-1 was transcribed in uninduced and induced cells from a single initiation site preceded by a TATA box, with the possible use of an additional TATA box and initiation site in uninduced cells. In induced cells, vIRF-2 was transcribed from a single major initiation site preceded by a TATA box, and vIRF-4 was expressed from two sites each preceded by a TATA box. Transcripts for these genes were insufficiently abundant in uninduced cells to map the 5'-ends. vIRF-3 lacks an obvious TATA box and exhibited heterogeneous 5'-ends in uninduced and induced cells. These data clarify and extend our understanding of the structure and transcription of the HHV-8 vIRF genes.
Starting Page	1
Ending Page	4
Page Count	4
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.microbiologyresearch.org/docserver/fulltext/jgv/84/6/1471.pdf?accname=guest&checksum=3DCDC81CC5FAF6AF376B5690A00D61B7&expires=1540904094&id=id
Alternate Webpage(s)	http://www.microbiologyresearch.org/docserver/fulltext/jgv/84/6/1471.pdf?accname=guest&checksum=33FAA007273116B21DC9FD24C0F97865&expires=1544561297&id=id
PubMed reference number	12771416v1
Volume Number	84
Part	6
Journal	The Journal of general virology
Language	English
Access Restriction	Open
Subject Keyword	Cultured Cell Line Exons Genetic Heterogeneity Herpesviridae Human herpes simplex virus Interferon Regulatory Factors Lysis Polyadenylation RNA Splicing TATA Box Transcript Transcription Initiation Transcription, Genetic human herpesvirus 8 promoter
Content Type	Text
Resource Type	Article

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