NDLI: spoIID operon of Bacillus subtilis: cloning and sequence.

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spoIID operon of Bacillus subtilis: cloning and sequence.

Content Provider	Semantic Scholar
Author	López-Díaz, Isabel Clarke, Siân E. Mandelstam, J. E.
Copyright Year	1986
Abstract	The locus spoIID, involved in the sporulation of Bacillus subtilis, was cloned into derivatives of the temperate phage phi 105. Two recombinant phages were obtained which contain chromosomal DNA covering 1.6 kbp. They are both able to complement mutations spo-68 and spo-298. These mutations, which were believed to be in different loci, spoIID and spoIIC respectively, were shown to be closely linked, and both map at the position assigned to spoIID on the genetic map of B. subtilis. The sequence of 1656 bp carrying the spoIID locus was determined. Only one open reading frame was found; this codes for a protein of 343 amino acids. It is preceded by a ribosome binding site and possible recognition sequences for sigma 32- and sigma 29-RNA polymerases. Studies of the locus by means of integrational plasmid vectors defined the outer limits of the transcriptional unit. These results are completely compatible with the sequence data. The combination of sequence and mapping and the information obtained by the use of integrational plasmids confirm that the spoIID locus functions as a monocistronic operon.
Starting Page	44
Ending Page	53
Page Count	10
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.microbiologyresearch.org/docserver/fulltext/micro/132/2/mic-132-2-341.pdf?accname=guest&checksum=044FFCA83AB9435A8E9A9F43E9126DC2&expires=1542224889&id=id
Alternate Webpage(s)	http://www.microbiologyresearch.org/docserver/fulltext/micro/132/2/mic-132-2-341.pdf?accname=guest&checksum=4A685236BDDFC5A5E44279BB6D89C771&expires=1540501874&id=id
PubMed reference number	3011962v1
Volume Number	132
Issue Number	2
Journal	Journal of general microbiology
Language	English
Access Restriction	Open
Subject Keyword	Amino Acids Bacillus subtilis Bacteriophage phi 6 Bacteriophages Clone Cells Complement System Proteins Mutation Open Reading Frames Operon PersonNameUse - assigned Reading Frames (Nucleotide Sequence) Recombinants Ribosomes Tacrolimus Binding Proteins Transcription, Genetic sporulation
Content Type	Text
Resource Type	Article

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