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Analysis of cartilage matrix fixed charge density and three-dimensional morphology via contrast-enhanced microcomputed tomography.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Palmer, Ashley W. Guldberg, Robert Erling Levenston, Marc |
| Copyright Year | 2006 |
| Abstract | Small animal models of osteoarthritis are often used for evaluating the efficacy of pharmacologic treatments and cartilage repair strategies, but noninvasive techniques capable of monitoring matrix-level changes are limited by the joint size and the low radiopacity of soft tissues. Here we present a technique for the noninvasive imaging of cartilage at micrometer-level resolution based on detecting the equilibrium partitioning of an ionic contrast agent via microcomputed tomography. The approach exploits electrochemical interactions between the molecular charges present in the cartilage matrix and an ionic contrast agent, resulting in a nonuniform equilibrium partitioning of the ionic contrast agent reflecting the proteoglycan distribution. In an in vitro model of cartilage degeneration we observed changes in x-ray attenuation magnitude and distribution consistent with biochemical and histological analyses of sulfated glycosaminoglycans, and x-ray attenuation was found to be a strong predictor of sulfated glycosaminoglycan density. Equilibration with the contrast agent also permits direct in situ visualization and quantification of cartilage surface morphology. Equilibrium partitioning of an ionic contrast agent via microcomputed tomography thus provides a powerful approach to quantitatively assess 3D cartilage composition and morphology for studies of cartilage degradation and repair. |
| Starting Page | 999 |
| Ending Page | 1005 |
| Page Count | 7 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.pnas.org/content/103/51/19255.full.pdf |
| PubMed reference number | 17158799v1 |
| Volume Number | 103 |
| Issue Number | 51 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Animal Model Contrast Media Degenerative polyarthritis Diagnostic radiologic examination Equilibrium License Micron Pharmacology Proteoglycan Quantitation Structure of costal cartilage Tomography, Emission-Computed X-Ray Computed Tomography X-Ray Microtomography soft tissue |
| Content Type | Text |
| Resource Type | Article |
