Loading...
Please wait, while we are loading the content...
Similar Documents
Fractionation by differential and zonal centrifugation of spheroplasts prepared from a glucose-repressed fission yeast Schizosaccharomyces pombe 972h-.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Poole, Robert K. Lloyd, David |
| Copyright Year | 1976 |
| Abstract | A method is described for the preparation of spheroplasts in high yield from Schizosaccharomyces pombe, by treating cells grown in the presence of glucose and deoxyglucose with snail digestive enzymes. Gentle disruption of such spheroplasts yielded homogenates, from which marker enzymes for nuclei (NAD pyrophosphorylase) and mitochondria (cytochrome c oxidase activity and spectroscopically-detectable cytochromes a + a3) could be quantitatively sedimented by low-speed centrifugation. In contrast to previous findings with Saccharomyces carlsbergensis, cytochrome c oxidase and another mitochondrial enzyme, succinate dehydrogenase, were completely sedimentable by zonal centrifugation in sucrose gradients in the presence of either 2 mM-MgCl2 or 0-4 mM-EDTA. Mitochondria were apparently smaller and of lower buoyant density in gradients containing EDTA. The bulk of the total units of malate dehydrogenase and NADH; cytochrome c oxidoreductase sedimented with mitochondria, whereas NADPH: cytochrome c oxidoreductase was located in fractions containing no mitochondria. The distributions of mitochondrial enzymes were heterogeneous in populations of mitochondria separated on the basis of size or density. The possible origins of mitochondrial heterogeneity in extracts of S. pombe are discussed with special reference to changes in the enzyme activities of cells during the cell cycle. |
| Starting Page | 1 |
| Ending Page | 6 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.microbiologyresearch.org/docserver/fulltext/micro/93/2/mic-93-2-241.pdf?accname=guest&checksum=05E408C33604DB759A88BCCA398FADF1&expires=1540496390&id=id |
| PubMed reference number | 180235v1 |
| Volume Number | 93 |
| Issue Number | 2 |
| Journal | Journal of general microbiology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | CYTOCHROME C OXIDASE Cellular Phone Centrifugation Centrifugation, Zonal Cytochrome a Deoxyglucose Digestion Edetic Acid Gastrointestinal system Genetic Heterogeneity Glucose Magnesium Chloride Mitochondria NADH National origin Nicotinamide adenine dinucleotide (NAD) Nicotinamide-Nucleotide Adenylyltransferase Schizosaccharomyces pombe Small Spheroplasts Succinate Dehydrogenase Succinates Sucrose malate |
| Content Type | Text |
| Resource Type | Article |
