Morphine enhances interleukin-12 and the production of other pro-inflammatory cytokines in mouse peritoneal macrophages.

Content Provider	Semantic Scholar
Author	Peng, Xiaohui Mosser, David M. Adler, Martin W. Rogers, Thomas J. Meissler, Joseph J. Eisenstein, Toby K.
Copyright Year	2000
Abstract	In this study we investigated the capacity of morphine to modulate expression of cytokines in peritoneal macrophages. Mice were implanted subcutaneously with a 75-mg morphine slow-release pellet, and 48 h later resident peritoneal macrophages were harvested. Control groups received placebo pellets, naltrexone pellets, or morphine plus naltrexone pellets. Adherent cells were stimulated with lipopolysaccharide (LPS: 10 microg/mL) plus interferon-gamma (IFN-gamma: 100 units/mL) to induce cytokine production. After 24 h RNA was extracted for analysis of cytokine mRNA levels by reverse transcriptase-polymerase chain reaction, or supernatants were collected after 48 h for determination of cytokine production by enzyme-linked immunosorbent assay (ELISA). Morphine enhanced mRNA expression of interleukin (IL)-12 p40 and tumor necrosis factor alpha (TNF-alpha) compared with controls, whereas IL-10 levels were unchanged by drug treatment. ELISA data showed that both IL-12 p40 and p70 were increased by morphine. The enhancement of IL-12 at both the mRNA and protein levels was antagonized by naltrexone, indicating that the modulation of this cytokine by morphine is via a classic opioid receptor. These results are particularly interesting in light of our previous observation that 48 h after morphine pellet implantation, the peritoneal cavity is colonized with gram-negative and other enteric bacteria. The enhancement of IL-12 by morphine might be related to morphine-induced sepsis.
Starting Page	9
Ending Page	21
Page Count	13
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.jleukbio.org/content/68/5/723.full.pdf
PubMed reference number	11073113v1
Volume Number	68
Issue Number	5
Journal	Journal of leukocyte biology
Language	English
Access Restriction	Open
Subject Keyword	Dental caries Enzyme-Linked Immunosorbent Assay Implants Interferon Type II Interleukin-12 Interleukins Lipopolysaccharides Macrophages, Peritoneal Morphine Naltrexone Neoplasms Opioid Receptor Oral Pellet Peritoneal Cavity RNA RNA-Directed DNA Polymerase Recombinant Interferon-gamma Reverse Transcriptase Polymerase Chain Reaction Sepsis Tumor Necrosis Factors cytokine gram
Content Type	Text
Resource Type	Article