NDLI: Cloning and characterization of a novel dehydrin gene, SiDhn2, from Saussurea involucrata Kar. et Kir.

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Cloning and characterization of a novel dehydrin gene, SiDhn2, from Saussurea involucrata Kar. et Kir.

Content Provider	Semantic Scholar
Author	Qiu, Honglin Zhang, Lin-Hua Liu, Chao Wang, Aiying Liu, Hai-Liang Zhu, Jianbo
Copyright Year	2013
Abstract	Saussurea involucrata Kar. et Kir. is a hardy dicotyledonous plant capable of tolerating severe abiotic stress conditions. In a previous study, we created a cDNA library to determine what factors are associated with the cold acclimation response in S. involucrata. From this, a full-length cDNA of a dehydrin-like gene (SiDhn2) was obtained by RT-PCR. The SiDhn2 gene was characterized in this study. The full-length SiDhn2 cDNA comprised 693 bp containing an open reading frame of 345 bp specifying a protein of 115 amino acids. An alignment of the deduced amino acid sequence showed that SiDhn2 shared 55 % identity with two Brassica dehydrins. Agrobacterium tumefaciens was used to transform RD29A:SiDhn2 and 35S:SiDhn2 constructs into tobacco to investigate the germination and resistance to freezing and drought stress of transgenic plants. The RD29A:SiDhn2 transgenic plants showed greater resistance to freezing and drought stress than 35S:SiDhn2 transgenic plants or the wild-type. This study demonstrates that SiDhn2 confers cold hardiness and drought resistance, and may be a candidate resistance gene for genetic improvement of crops to increase stress resistance.
Starting Page	707
Ending Page	718
Page Count	12
File Format	PDF HTM / HTML
DOI	10.1007/s11103-013-0164-7
Alternate Webpage(s)	http://or.nsfc.gov.cn/bitstream/00001903-5/44879/1/1000007676614.pdf
PubMed reference number	24337866
Alternate Webpage(s)	https://doi.org/10.1007/s11103-013-0164-7
Journal	Medline
Volume Number	84
Journal	Plant Molecular Biology
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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