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Re-engineering a NiFe hydrogenase to increase the H2 production bias while maintaining native levels of O2 tolerance† †Electronic supplementary information (ESI) available: Experimental protocol details and Fig. S1–S7. See DOI: 10.1039/c6cc00515b Click here for additional data file.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Flanagan, Lindsey A. Wright, John Jessen Roessler, Maxie M. Moir, James W. B. Parkin, Alison |
| Copyright Year | 2016 |
| Abstract | Naturally occurring oxygen tolerant NiFe membrane bound hydrogenases have a conserved catalytic bias towards hydrogen oxidation which limits their technological value. We present an Escherichia coli Hyd-1 amino acid exchange that apparently causes the catalytic rate of H2 production to double but does not impact the O2 tolerance. |
| Starting Page | 9133 |
| Ending Page | 9136 |
| Page Count | 4 |
| File Format | PDF HTM / HTML |
| DOI | 10.1039/c6cc00515b |
| PubMed reference number | 27055899 |
| Journal | Medline |
| Volume Number | 52 |
| Alternate Webpage(s) | http://eprints.whiterose.ac.uk/98411/1/ChemComm_Parkin_Submission_correction_FINALVERSION_black.pdf |
| Alternate Webpage(s) | https://doi.org/10.1039/c6cc00515b |
| Journal | Chemical communications |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |