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Polyamine regulation of heat-shock-induced spermidine N1-acetyltransferase activity.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Fuller, David J. M. Carper, Stephen W. Clay, Laverne Chen, Jianqiang Gerner, Eugene W. |
| Copyright Year | 1990 |
| Abstract | The enzyme spermidine/spermine N1-acetyltransferase (N1-SAT) is rapidly induced by heat shock in CHO and A549 cells, with activity declining by 24 h. Depletion of intracellular polyamines by alpha-difluoromethylornithine, an inhibitor of ornithine decarboxylase, blocks this induction. Re-addition of putrescine to these cultures restores the response to heat shock, with a concomitant increase in intracellular N1-acetylspermidine. Diaminopropane is more than twice as effective as the naturally occurring diamine putrescine, suggesting that the propylamine moiety of spermidine is involved in the regulation of N1-SAT induction. Inhibitor studies indicate transcriptional activation and that the enzyme has an apparent half-life of 30-60 min. A second heat shock rapidly inhibits induced N1-SAT activity, which decays with a half-life of 2-3 min. Despite its induction by heat, N1-SAT is not a stable enzyme, suggesting that the activity observed is not due to a modification of an existing peptide, but is due to a transcriptional event, which may justify the inclusion of this enzyme in the family of heat-shock proteins. |
| Starting Page | 1 |
| Ending Page | 5 |
| Page Count | 5 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.biochemj.org/content/ppbiochemj/267/3/601.full.pdf |
| PubMed reference number | 2111132v1 |
| Volume Number | 267 |
| Issue Number | 3 |
| Journal | The Biochemical journal |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Carboxy-Lyases Diamine oxidase:ACnc:Pt:Ser/Plas:Qn Diamines Difluoromethylornithine Eflornithine Heat-Shock Response Ornithine Decarboxylase Polyamines Propylamines Putrescine Shock Spermine Synthase Transcription, Genetic Transcriptional Activation spermidine type I interferon receptor |
| Content Type | Text |
| Resource Type | Article |
