Loading...
Please wait, while we are loading the content...
Similar Documents
Purification and properties of two neutral proteinases from Aspergillus nidulans.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Ansari, Hassan Stevens, Lk |
| Copyright Year | 1983 |
| Abstract | Two proteinases have been purified from mycelial extracts of Aspergillus nidulans. Both enzymes have pH optima between 6.5 and 7.5 and are inhibited by phenylmethane sulphonyl fluoride and by di-isopropyl fluorophosphate. The molecular weights and isoelectric points of proteinase I and proteinase II are 30 900 and 30 000, and 4.6 and 4.3, respectively. Both enzymes have a similar range of substrate specificities. The principal differences in their properties are that proteinase I is sensitive to inhibition by 1 mM mercurials whereas proteinase II is not appreciably inhibited at this concentration, and that proteinase I is much more sensitive to denaturation by urea, guanidine hydrochloride and sodium dodecyl sulphate. |
| File Format | PDF HTM / HTML |
| DOI | 10.1099/00221287-129-6-1637 |
| PubMed reference number | 6355383 |
| Journal | Medline |
| Volume Number | 129 |
| Issue Number | 6 |
| Alternate Webpage(s) | http://www.microbiologyresearch.org/docserver/fulltext/micro/129/6/mic-129-6-1637.pdf?accname=guest&checksum=826100106C5061E009F6E9EC9A119CD8&expires=1543293722&id=id |
| Alternate Webpage(s) | https://www.microbiologyresearch.org/docserver/fulltext/micro/129/6/mic-129-6-1637.pdf?accname=guest&checksum=E172390DDC4D3F04D55A8461A0057EF8&expires=1548195207&id=id |
| Alternate Webpage(s) | http://www.microbiologyresearch.org/docserver/fulltext/micro/129/6/mic-129-6-1637.pdf?accname=guest&checksum=DFFD4BBF791C4B58FEDE90A7642240AB&expires=1541242925&id=id |
| Alternate Webpage(s) | https://www.microbiologyresearch.org/docserver/fulltext/micro/129/6/mic-129-6-1637.pdf?accname=guest&checksum=DA2422669F3F5F3162821D3F78DE6606&expires=1547096297&id=id |
| Alternate Webpage(s) | http://www.microbiologyresearch.org/docserver/fulltext/micro/129/6/mic-129-6-1637.pdf?accname=guest&checksum=ED656704C6A9ACAF8550BF84DDF6FE16&expires=1541536435&id=id |
| Alternate Webpage(s) | http://www.microbiologyresearch.org/docserver/fulltext/micro/129/6/mic-129-6-1637.pdf?accname=guest&checksum=1DD91730A779A0FA69B71AE011C94E62&expires=1540617512&id=id |
| Alternate Webpage(s) | https://doi.org/10.1099/00221287-129-6-1637 |
| Journal | Journal of general microbiology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
