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Enhanced Inhibition of Human Anti-Gal Antibody Binding to Mammalian Cells by Synthetic α-Gal Epitope Polymers
| Content Provider | Semantic Scholar |
|---|---|
| Author | Wang, Jianq Qiang Chen, Xiao Dong Zhang, Wei Zacharek, Sima Chen, Yongsheng Wang, Peng George |
| Copyright Year | 1999 |
| Abstract | Neoglycopolymers of polyacrylamide backbone conjugated with varying densities of Galα1−3Galβ1−4Glcβ trisaccharide epitopes (α-Gal epitopes) were designed and synthesized to study the inhibition of the binding of human natural anti-Gal antibodies to either α-Gal-containing glycoproteins or α-Gal antigens on the surface of mammalian cells. An inhibition ELISA using mouse laminin and a flow cytometry assay using pig kidney cells (PK15) were established to determine the binding affinity of the synthesized polymers. In comparison to the α-Gal monomer (Galα1−3Galβ1−4GlcNHAcβ), the α-Gal polymers dramatically enhanced the inhibition of human anti-Gal antibodies (IgG, IgM, and IgA) binding to mouse laminin or mammalian cells. Increases of 7.8 × 103- and 5.0 × 104 -fold in inhibitory potential of polymer 7C to IgA and IgM (with IC50s of 7.0 and 5.6 nM respectively) were observed over the monomer in inhibition ELISA. The results also indicated that binding enhancement of α-Gal polymers is greater for anti-Gal IgA a... |
| Starting Page | 8174 |
| Ending Page | 8181 |
| Page Count | 8 |
| File Format | PDF HTM / HTML |
| DOI | 10.1021/ja990219h |
| Alternate Webpage(s) | http://chemgroups.ucdavis.edu/~chen/publication%20files/No-4.pdf |
| Alternate Webpage(s) | https://doi.org/10.1021/ja990219h |
| Volume Number | 121 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
