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Flagellated but not hyperfimbriated Salmonella enterica serovar Typhimurium attaches to and forms biofilms on cholesterol-coated surfaces.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Crawford, Robert Wilson Reeve, Kristin E. Gunn, John S. |
| Copyright Year | 2010 |
| Abstract | The asymptomatic, chronic carrier state of Salmonella enterica serovar Typhi occurs in the bile-rich gallbladder and is frequently associated with the presence of cholesterol gallstones. We have previously demonstrated that salmonellae form biofilms on human gallstones and cholesterol-coated surfaces in vitro and that bile-induced biofilm formation on cholesterol gallstones promotes gallbladder colonization and maintenance of the carrier state. Random transposon mutants of S. enterica serovar Typhimurium were screened for impaired adherence to and biofilm formation on cholesterol-coated Eppendorf tubes but not on glass and plastic surfaces. We identified 49 mutants with this phenotype. The results indicate that genes involved in flagellum biosynthesis and structure primarily mediated attachment to cholesterol. Subsequent analysis suggested that the presence of the flagellar filament enhanced binding and biofilm formation in the presence of bile, while flagellar motility and expression of type 1 fimbriae were unimportant. Purified Salmonella flagellar proteins used in a modified enzyme-linked immunosorbent assay (ELISA) showed that FliC was the critical subunit mediating binding to cholesterol. These studies provide a better understanding of early events during biofilm development, specifically how salmonellae bind to cholesterol, and suggest a target for therapies that may alleviate biofilm formation on cholesterol gallstones and the chronic carrier state. |
| Starting Page | 1116 |
| Ending Page | 1117 |
| Page Count | 2 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://jb.asm.org/content/192/12/2981.full.pdf |
| PubMed reference number | 20118264v1 |
| Alternate Webpage(s) | https://doi.org/10.1128/JB.01620-09 |
| DOI | 10.1128/JB.01620-09 |
| Journal | Journal of bacteriology |
| Volume Number | 192 |
| Issue Number | 12 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Anabolism Bacterial Fimbria Bile fluid Biliary calculi Cholesterol gallstones Cytoskeletal Filaments Enzyme-Linked Immunosorbent Assay Flagella Forty Nine Leptospira interrogans serovar Grippotyphosa Ab:Titr:Pt:Ser:Qn:Comp fix Mastigophora Microbial Biofilms Salmonella enterica subsp. enterica serovar enteritidis Serotype Specimen Source Codes - Tube bacterial-type flagellum filament biofilm formation mutant |
| Content Type | Text |
| Resource Type | Article |
